Millipore Sigma Vibrant Logo

MAB1398Z Anti-PECAM-1 Antibody, clone 2H8, Azide Free

View Products on Sigmaaldrich.com
MAB1398Z
100 µg  
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
Solicitar precio
Disponibilidad a confirmar
Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      Ofertas especiales

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, CaEM, FC, ICC, IF, IHC, IP, Neutralization AssayAHmPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1398Z
      Replaces04-1074
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-PECAM-1 Antibody, clone 2H8, Azide Free
      Alternate Names
      • CD31
      • PECAM-1
      • Platelet endothelial cell adhesion molecule
      Background InformationCD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets.

      CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
      Product Information
      FormatPurified
      Control
      • Mouse cell lung tissue
      PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PECAM-1 Antibody, clone 2H8, Azide Free detects level of PECAM-1 & has been published & validated for use in Elecron Microscopy, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Neutralization Assay.
      Key Applications
      • Electron Microscopy
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Neutralization Assay
      Application NotesImmunofluorescence Analysis: Representative lots immunostained endothelial blood vessel cells in 4% paraformaldehyde-fixed frozen tumor sections from mice and dogs as a means for assessing lymph vessel density in tumor xenografts by fluorescent immunohistochemistry (Donat, U., et al. (2012). PLoS One. 7(9):e45942; Gentschev, I., et al. (2012). PLoS One.7(5):e37239).
      Immunofluorescence Analysis: A representative lot immunostained endothelial cells in formalin-fixed, paraffin-embedded murine peritoneum sections by fluorescent immunohistochemistry (Tanabe, K., et al. (2007). Kidney Int. 71(3):227-238).
      Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed endothelial cells differentiated from murine ESCs in cultures (Purpura, K.A., et al. (2008). Stem Cells. 26(11):2832-2842).
      Immunocytochemistry Analysis: Clone 2H8 immunostained 3% paraformaldehyde-fixed, 0.5% NP-40-permeabilized COS-7 transfectants expressing murine, but not human, CD31 by fluorescent immunocytochemistry (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
      Neutralization Analysis: A representative lot, when injected intravenously in mice, blocked leukocyte emigration in response to thioglycollate injection (Bogen, S., et al. (1994). J. Exp. Med. 179(3):1059-1064).
      Neutralization Analysis: A representative lot blocked murine PECAM-mediated aggregation of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
      Flow Cytometry Analysis: A representative lot detected the expression of exogenously transfected murine CD31 on the surface of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
      Flow Cytometry Analysis: Clone 2H8 detected CD31 immunoreactivity on the surface of various murine B- and T-cell lines (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed murine, but not human, CD31 from COS-7 transfectants (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
      Immunoprecipitation Analysis: Clone 2H8 immunoprecipitated CD31 from murine CTLL T lymphocytes and Sp2/0 B lymphocytes (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
      Electron Microscopy Analysis: Clone 2H8 was biotinylated and immunostained lymphocytes in 0.01% glutaraldehyde-/2% formaldehyde-fxed frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
      Immunohistochemistry Analysis: Clone 2H8 immunostained lymphocytes adherent to the sinusoidal and postcapillary venular endothelium of frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
      Biological Information
      ImmunogenD10.G4.1 murine T cells (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843 -854).
      EpitopeExtracellular domain.
      Clone2H8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostArmenian Hamster
      SpecificityReact s with the 130-140 kDa protein, PECAM-1 found on mouse monocytes, platelets, granulocytes and endothelial cells. 2H8 effectively inhibits transmigration of activated polymophonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonis - 2H8 blocks acute inflammation.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Canine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pecam
      • Pecam-1
      • Pecam1
      Purification MethodProtein A Purfied
      UniProt Number
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Número de referencia GTIN
      MAB1398Z 04053252399893