Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Fast filtration and highly reproducible performance
Use in fixed-angle rotors for 1.5 mL tubes
Applications
Removal of particles and precipitates from aqueous and some solvent-based samples
Ideal for use with protein and nucleic acid solutions
Ultrafree®-MC (0.5 mL) and Ultrafree®-CL (2 mL) centrifugal devices are single-use, disposable filters used for removing particulates from aqueous biological solutions. These devices are available in two processing volumes with a range of microporous membranes (from 0.1 to 5.0 µm) for fast filtration and highly reproducible performance. Pre-sterilized units are also available. Use in fixed-angle rotors for 1.5 mL tubes (-MC) and 15 mL tubes (–CL).
Specifications
Volumes
Maximum Initial Sample Volume, mL
0.5 mL
Hold-up Volume
5 µL
Centrifugal Force
Fixed-angle microfuge rotor w/maximum 12,000 x g
Materials
Membrane
Hydrophilic PVDF or hydrophilic PTFE
Device
Polypropylene
Dimensions
Active Membrane Area
0.2 cm²
Diameter
10.6 mm
Length
45 mm
Ordering Information
Documentation
FAQ
Question
Answer
What are the hold-up volumes of the Ultrafree MC and CL units?
The hold-up volumes are as follows:
Device
Hold-up
MC
5ul
CL
30ul
What are the membrane types available in Ultrafree MC or Ultrafree CL?
Both the Ultrafree MC and Ultrafree CL are available with the Durapore Microporous Membrane. This is a low protein binding membrane available in 0.1u, 0.22u, 0.45 and 0.65u pore sizes.
What are the dimensions of the Ultrafree MC?
Ultrafree MC has a diameter of 10.6 mm and a length of 45 mm.
Is the Ultrafree MC/CL available with a PTFE membrane?
Yes, the Ultrafree MC/CL is available is with a either a 0.22um or 0.45um "LCR" hydrophilic PTFE membrane. These products are available in packages of twenty-five units under the following catalog numbers: 0.22 micron - UFC3 0LG 25 and 0.45 micron - UFC3 0LH 25.
Can I purchase the 1.5mL Ultrafree MC microcentrifuge tubes separately?
Yes, the 1.5mL microcentrifuge tubes which are sold with the Ultrafree MC filter unit are available separately under product number UFC3000TB.
Is the Ultrafree MC sterile or autoclavable?
It is not autoclavable. However, it is offered sterile in the 0.22 mm (catalog number UFC30GV0S), 0.45 mm (catalog number UFC30HV0S), 0.65 mm (catalog number UFC30DV0S), and 10 kDa (catalog number UFC3LGC0S).
