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569397 Staurosporine, Streptomyces sp.

Staurosporine, CAS 62996-74-, is a cell-permeable, potent, reversible, ATP-competitive inhibitor of protein kinases (IC₅₀ = 7, 20, 1.3, 0.7, & 8.5 nM for PKA, CAMK, MLCK, PKC, & PKG, respectively).

Staurosporine, Streptomyces sp. MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: MLCK Inhibitor I, PKA Inhibitor II

Primary Target: PKA
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569397
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
62996-74-1C₂₈H₂₆N₄O₃

Pricing & Availability

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569397-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewA potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases. Inhibits protein kinase A (IC50 = 7 nM), CaM kinase (IC50 = 20 nM), myosin light chain kinase (IC50 = 1.3 nM), protein kinase C (IC50 = 700 pM), and protein kinase G (IC50 = 8.5 nM). Also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation. Induces apoptosis in human malignant glioma cell lines. Arrests normal cells at the G1 checkpoint. A 1 mM (100 µg/214 µl) solution of Staurosporine (Cat. No. 569396) in DMSO is also available.
      Catalogue Number569397
      Brand Family Calbiochem®
      SynonymsMLCK Inhibitor I, PKA Inhibitor II
      References
      ReferencesCouldwell, W.T., et al. 1994. FEBS Lett. 345, 43.
      Nishimura, H. and Simpson, I.A. 1994. Biochem. J. 302, 271.
      Bruno, S., et al. 1992. Cancer Res. 52, 470.
      Kiss, Z. and Deli, E. 1992. Biochem. J. 288, 853.
      Vitale, M.L., et al. 1992. Neuroscience 51, 463.
      Hoffman, R. and Newland, E.S. 1991. Cancer Chemother. Pharmacol. 28, 102.
      Oka, S., et al. 1986. Agric. Biol. Chem. 50, 2723.
      Product Information
      CAS number62996-74-1
      ATP CompetitiveY
      FormWhite to pale yellow solid
      Hill FormulaC₂₈H₂₆N₄O₃
      Chemical formulaC₂₈H₂₆N₄O₃
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetPKA
      Primary Target IC<sub>50</sub>7 nM, 20 nM, 1.3 nM, 700 pM, and 8.5 nM against protein kinase A , CaM kinase, myosin light chain kinase, protein kinase C, and protein kinase G, respectively
      Purity≥99% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      569397-100UG 07790788057855

      Documentation

      Staurosporine, Streptomyces sp. SDS

      Title

      Safety Data Sheet (SDS) 

      Staurosporine, Streptomyces sp. Certificates of Analysis

      TitleLot Number
      569397

      References

      Reference overview
      Couldwell, W.T., et al. 1994. FEBS Lett. 345, 43.
      Nishimura, H. and Simpson, I.A. 1994. Biochem. J. 302, 271.
      Bruno, S., et al. 1992. Cancer Res. 52, 470.
      Kiss, Z. and Deli, E. 1992. Biochem. J. 288, 853.
      Vitale, M.L., et al. 1992. Neuroscience 51, 463.
      Hoffman, R. and Newland, E.S. 1991. Cancer Chemother. Pharmacol. 28, 102.
      Oka, S., et al. 1986. Agric. Biol. Chem. 50, 2723.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Protein Kinase Assay and Detection Kits Brochure
      Tools and Tips for Analyzing Apoptosis

      Citations

      Title
    • Joao B. Oliveira, et al. (2007) NRAS mutation causes a human autoimmune lymphoproliferative syndrome. Procedings of the National Academy of Science 104, 8953-8958.
    • Siva Kumar Kolluri, et al. (2005) The R-enantiomer of the nonsteroidal antiinflammatory drug etodolac binds retinoid X receptor and induces tumor-selective apoptosis. Procedings of the National Academy of Science 102, 2525-2530.
    • Rick B. Vega, et al. (2004) Protein Kinases C and D Mediate Agonist-Dependent Cardiac Hypertrophy through Nuclear Export of Histone Deacetylase 5. Molecular and Cellular Biology 24, 8374-8385.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-July-2014 JSW
      SynonymsMLCK Inhibitor I, PKA Inhibitor II
      DescriptionA potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases. Inhibits CaM kinase (IC50 = 20 nM), myosin light chain kinase (IC50 = 1.3 nM), protein kinase A (IC50 = 7 nM), protein kinase C (IC50 = 0.7 nM), and protein kinase G (IC50 = 8.5 nM). Also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation. Induces apoptosis in human malignant glioma cell lines. Arrests normal cells at the G1 checkpoint.
      FormWhite to pale yellow solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number62996-74-1
      Chemical formulaC₂₈H₂₆N₄O₃
      Structure formulaStructure formula
      Purity≥99% by HPLC
      SolubilityDMSO (5 mg/ml). Further dilute with aqueous buffers just prior to use.
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
      Toxicity Standard Handling
      ReferencesCouldwell, W.T., et al. 1994. FEBS Lett. 345, 43.
      Nishimura, H. and Simpson, I.A. 1994. Biochem. J. 302, 271.
      Bruno, S., et al. 1992. Cancer Res. 52, 470.
      Kiss, Z. and Deli, E. 1992. Biochem. J. 288, 853.
      Vitale, M.L., et al. 1992. Neuroscience 51, 463.
      Hoffman, R. and Newland, E.S. 1991. Cancer Chemother. Pharmacol. 28, 102.
      Oka, S., et al. 1986. Agric. Biol. Chem. 50, 2723.
      Citation
    • Joao B. Oliveira, et al. (2007) NRAS mutation causes a human autoimmune lymphoproliferative syndrome. Procedings of the National Academy of Science 104, 8953-8958.
    • Siva Kumar Kolluri, et al. (2005) The R-enantiomer of the nonsteroidal antiinflammatory drug etodolac binds retinoid X receptor and induces tumor-selective apoptosis. Procedings of the National Academy of Science 102, 2525-2530.
    • Rick B. Vega, et al. (2004) Protein Kinases C and D Mediate Agonist-Dependent Cardiac Hypertrophy through Nuclear Export of Histone Deacetylase 5. Molecular and Cellular Biology 24, 8374-8385.
      		•

      Related Products & Applications

      Categories

      Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Protein Phosphorylation / Dephosphorylation > Protein Kinase C (PKC) Inhibitors
      Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Protein Phosphorylation / Dephosphorylation > Protein Kinase A (PKA; cAMP-Dependent Protein Kinase) Inhibitors
      Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Protein Phosphorylation / Dephosphorylation > Protein Kinase G (PKG; cGMP-Dependent Protein Kinase) Inhibitors