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70663 Proteinase K, Lyophilized

Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA.

Proteinase K, Lyophilized MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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70663
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Overview

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      		 Plastic ampoule 100 mg
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      Description
      OverviewProteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/ml for protein removal and enzyme inactivation, and up to 2 mg/ml for tissue treatment. The Proteinase K, Lyophilized powder can be prepared as a 20 mg/ml stock solution in water and stored in aliquots at -20°C. The enzyme is also available as a ready-to-use concentrated stock solution (600 mAU/ml) that is convenient for routine use in most applications. 1 mg of Proteinase K is the equivalent of 30 mAU (AU = Anson unit). The Novagen Proteinase K products are free of detectable DNase and RNase
      Catalogue Number70663
      Brand Family Novagen®
      References
      Product Information
      CAS number39450-01-6
      Unit of DefinitionOne AU (AU = Anson unit) is defined as the amount of enzyme that liberates 1.0 µmol (181 µg) of tyrosine from casein per minute at pH 7.5 at 37°C.
      Quality LevelMQ400
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38-42

      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation.
      S PhraseS: 22-24-26-36/37

      Do not breathe dust.
      Avoid contact with skin.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing and gloves.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Harmful
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70663-4 04055977257670

      Documentation

      Proteinase K, Lyophilized SDS

      Title

      Safety Data Sheet (SDS) 

      Proteinase K, Lyophilized Certificates of Analysis

      TitleLot Number
      70663

      Brochure

      Title
      High fidelity gene amplification
      The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

      Citations

      Title
    • Shannon M. Anderson, et al. (2007) New markers for murine memory B cells that define mutated and unmutated subsets. Journal of Experimental Medicine 204, 2103-2114.
    • Andrew A. Horwitz, et al. (2007) ATP-induced structural transitions in PAN, the proteasome-regulatory ATPase complex in Archaea. Journal of Biological Chemistry 282, 22921-22929.
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      User Protocols

      Title
      TB271 Proteinase K