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537059 Propidium Iodide - CAS 25535-16-4 - Calbiochem

Membrane impermeable DNA intercalator.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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537059
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
25535-16-4C₂₇H₃₄N₄I₂

Pricing & Availability

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537059-100MG
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      		 Glass bottle 100 mg
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      537059-50MG
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          Description
          OverviewMembrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).
          Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;
          Catalogue Number537059
          Brand Family Calbiochem®
          References
          ReferencesBelloc, F., et al. 1994. Cytometry 17, 59.
          Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
          Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
          de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
          Pollice, A.A., et al. 1992. Cytometry 13, 432.
          Product Information
          CAS number25535-16-4
          ATP CompetitiveN
          FormDark red solid
          Hill FormulaC₂₇H₃₄N₄I₂
          Chemical formulaC₂₇H₃₄N₄I₂
          ReversibleN
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Applications
          Biological Information
          Primary TargetMembrane impermeable DNA intercalator
          Sample TypeIntact Cells
          Physicochemical Information
          Cell permeableN
          Emission max.
          Excitation max.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSSF7949600
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          537059-100MG 04055977195897
          537059-50MG 04055977195910

          Documentation

          Propidium Iodide - CAS 25535-16-4 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Propidium Iodide - CAS 25535-16-4 - Calbiochem Certificates of Analysis

          TitleLot Number
          537059

          References

          Reference overview
          Belloc, F., et al. 1994. Cytometry 17, 59.
          Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
          Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
          de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
          Pollice, A.A., et al. 1992. Cytometry 13, 432.

          Brochure

          Title
          Caspases and other Apoptosis Related Tools Brochure

          Citations

          Title
        • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
        • Tirado, O.M., et al. 2005. Cancer Research 65, 9320.
          		•
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision29-August-2008 RFH
          DescriptionMembrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M-1cm-1 (493 nm); 55,700 M-1cm-1 (287 nm); 13,800 M-1cm-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.
          FormDark red solid
          CAS number25535-16-4
          RTECSSF7949600
          Chemical formulaC₂₇H₃₄N₄I₂
          Structure formulaStructure formula
          SolubilityH₂O. Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.
          Storage Protect from light
          +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Irritant
          ReferencesBelloc, F., et al. 1994. Cytometry 17, 59.
          Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
          Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
          de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
          Pollice, A.A., et al. 1992. Cytometry 13, 432.
          Citation
        • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
        • Tirado, O.M., et al. 2005. Cancer Research 65, 9320.
          		•