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524400 Phorbol-12-myristate-13-acetate - CAS 16561-29-8 - Calbiochem

Phorbol-12-myristate-13-acetate, CAS 16561-29-8, acts as an extremely potent mouse skin tumor promoter. Activates protein kinase C in vivo and in vitro at nM concentrations.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: PMA, TPA, PKC Activator I

Primary Target: Protein Kinase C
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524400
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
16561-29-8C₃₆H₅₆O₈

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524400-10MG
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      524400-1MG
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          524400-5MG
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              Description
              OverviewMost commonly-used phorbol ester. Extremely potent mouse skin tumor promoter. Activates protein kinase C in vivo and in vitro, even at nM concentrations. Promotes the expression of inducible NOS in cultured hepatocytes. Activates Ca2+-ATPase and potentiates forskolin-induced cAMP formation. Inhibits apoptosis induced by the Fas antigen, but induces apoptosis in HL-60 promyelocytic leukemia cells. Its binding is reversible. Also available as a 10 mM solution in DMSO (Cat. No. 500582).
              Catalogue Number524400
              Brand Family Calbiochem®
              SynonymsPMA, TPA, PKC Activator I
              References
              ReferencesPowell, D.J., et al. 2003. Mol. Cell Biol. 23, 7794.
              Tepper, C.G., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8443.
              Oishi, K., and Yamaguchi, M. 1994. J. Cell. Biochem. 55, 168.
              Macfarlane, D.E., and O’Donnell, P.S. 1993. Leukemia 7, 1846.
              Ghandi, V.C. and Jones, D.J., 1992. Neuropharmacol. 31, 1101.
              Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
              Kontny, E., et al. 1992. Eur. J. Pharmacol. 227, 333.
              Zanaboni, P.B., et al. 1992. J. Appl. Physiol. 73, 2011.
              Saltis, J., et al. 1991. J. Biol. Chem. 266, 261.
              Beguinot, L., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 2774.
              Perchellet, J. 1985. Carcinogenesis 6, 567.
              Nishizuka, Y. 1984. Science 255, 1365.
              Mastro, A. 1982. Lymphokines 6, 263.
              Product Information
              CAS number16561-29-8
              ATP CompetitiveN
              FormWhite solid
              Hill FormulaC₃₆H₅₆O₈
              Chemical formulaC₃₆H₅₆O₈
              ReversibleY
              Structure formula ImageStructure formula Image
              Quality LevelMQ100
              Applications
              ApplicationPhorbol-12-myristate-13-acetate, CAS 16561-29-8, acts as an extremely potent mouse skin tumor promoter. Activates protein kinase C in vivo and in vitro at nM concentrations.
              Biological Information
              Primary TargetProtein Kinase C
              Purity≥98% by HPLC
              Physicochemical Information
              Cell permeableN
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSQH4377000
              Safety Information
              R PhraseR: 21-38

              Harmful in contact with skin.
              Irritating to skin.
              S PhraseS: 26-27-36-45

              In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
              Take off immediately all contaminated clothing.
              Wear suitable protective clothing.
              In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Harmful
              Storage -20°C
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Anhydrous DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.
              Packaging Information
              Packaged under inert gas Packaged under inert gas
              Transport Information
              Supplemental Information
              Specifications
              Global Trade Item Number
              Catalogue Number GTIN
              524400-10MG 04055977270952
              524400-1MG 04055977270945
              524400-5MG 04055977270976

              Documentation

              Phorbol-12-myristate-13-acetate - CAS 16561-29-8 - Calbiochem SDS

              Title

              Safety Data Sheet (SDS) 

              Phorbol-12-myristate-13-acetate - CAS 16561-29-8 - Calbiochem Certificates of Analysis

              TitleLot Number
              524400

              References

              Reference overview
              Powell, D.J., et al. 2003. Mol. Cell Biol. 23, 7794.
              Tepper, C.G., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8443.
              Oishi, K., and Yamaguchi, M. 1994. J. Cell. Biochem. 55, 168.
              Macfarlane, D.E., and O’Donnell, P.S. 1993. Leukemia 7, 1846.
              Ghandi, V.C. and Jones, D.J., 1992. Neuropharmacol. 31, 1101.
              Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
              Kontny, E., et al. 1992. Eur. J. Pharmacol. 227, 333.
              Zanaboni, P.B., et al. 1992. J. Appl. Physiol. 73, 2011.
              Saltis, J., et al. 1991. J. Biol. Chem. 266, 261.
              Beguinot, L., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 2774.
              Perchellet, J. 1985. Carcinogenesis 6, 567.
              Nishizuka, Y. 1984. Science 255, 1365.
              Mastro, A. 1982. Lymphokines 6, 263.

              Brochure

              Title
              Caspases and other Apoptosis Related Tools Brochure

              Citations

              Title
            • Cvetanovic, M., et al. 2006. Journal of Biological Chemistry 281, 20055.
            • Jafri, F., et al. 2006. Journal of Biological Chemistry 281, 19346.
            • Jiang, X., et al. 2005. Arteriosclerosis, Thrombosis, and Vascular Biology 25, 2515.
            • Yoon-Jin Lee, et al. 2005. Journal of Biological Chemistry 280, 18108.
              		•
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision19-October-2010 JSW
              SynonymsPMA, TPA, PKC Activator I
              DescriptionExtremely potent mouse skin tumor promoter. Activates protein kinase C (PKC) in vivo and in vitro, even at nanomolar concentrations. Promotes the expression of iNOS in cultured hepatocytes. Activates Ca2+-ATPase and potentiates forskolin-induced cAMP formation. Inhibits apoptosis induced by the Fas antigen, but induces apoptosis in HL-60 promyelocytic leukemia cells. Most commonly used phorbol ester.
              FormWhite solid
              Intert gas (Yes/No) Packaged under inert gas
              CAS number16561-29-8
              RTECSQH4377000
              Chemical formulaC₃₆H₅₆O₈
              Structure formulaStructure formula
              Purity≥98% by HPLC
              SolubilityDMSO (10 mg/ml). Further dilute with aqueous buffers just prior to use.
              Storage -20°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Anhydrous DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.
              Toxicity Harmful
              ReferencesPowell, D.J., et al. 2003. Mol. Cell Biol. 23, 7794.
              Tepper, C.G., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8443.
              Oishi, K., and Yamaguchi, M. 1994. J. Cell. Biochem. 55, 168.
              Macfarlane, D.E., and O’Donnell, P.S. 1993. Leukemia 7, 1846.
              Ghandi, V.C. and Jones, D.J., 1992. Neuropharmacol. 31, 1101.
              Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
              Kontny, E., et al. 1992. Eur. J. Pharmacol. 227, 333.
              Zanaboni, P.B., et al. 1992. J. Appl. Physiol. 73, 2011.
              Saltis, J., et al. 1991. J. Biol. Chem. 266, 261.
              Beguinot, L., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 2774.
              Perchellet, J. 1985. Carcinogenesis 6, 567.
              Nishizuka, Y. 1984. Science 255, 1365.
              Mastro, A. 1982. Lymphokines 6, 263.
              Citation
            • Cvetanovic, M., et al. 2006. Journal of Biological Chemistry 281, 20055.
            • Jafri, F., et al. 2006. Journal of Biological Chemistry 281, 19346.
            • Jiang, X., et al. 2005. Arteriosclerosis, Thrombosis, and Vascular Biology 25, 2515.
            • Yoon-Jin Lee, et al. 2005. Journal of Biological Chemistry 280, 18108.
              		•