Novagen® Competent Cells for Bacterial Protein Expression
Optimized for high yields of full-length, soluble protein
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Optimized for high yields of full-length, soluble protein Less
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Overview
When expressing recombinant proteins in E. coli, you need to obtain high yields of full-length, soluble protein. Our bacterial strains for protein expression include the best all-purpose strains and specialty strains for difficult-to-express proteins, all backed by unwavering technical support to ensure success. For ultimate convenience and reliability, Singles™ Competent Cells are provided in 50 μL volumes to eliminate the need to aliquot, freeze/thaw, or waste partially used vials. This saves time and money, minimizes contamination and ensures reliable cell performance.
Features & Benefits
- High yields of full-length, soluble protein
- Easy-to-use Singles™ format for greater convenience
- Selection of cell strains optimized for specific applications
Applications
Bacterial Protein Expression
Specifications
| Application Guide | ||
| Expression Strains | Feature | Target Application |
| DE3 Hosts | Lysogens of bacteriophage λDE3 that express T7 RNA polymerase | Protein induction from T7 expression vectors |
| (DE3) pLysS Hosts | Express T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction | No protein/cell death due to toxic target protein |
| BL21 Strains | Deficient in Lon and OmpT proteases that minimize protein degradation | General protein expression |
| Origami™ 2 and Rosetta-gami™ Strains | TrxB/gor- hosts that minimize protein reduction in cytoplasm | Insoluble protein/no activity |
| Tuner™ and Rosetta-gami™ B Strains | LacY- hosts that attenuate expression/titrate IPTG and minimize protein misfolding | Insoluble protein/no activity |
| Rosetta and Rosetta-gami™ Strains | Supply rare tRNAs for improved full-length protein expression | Truncated protein |
| HMS174 and NovaBlue Strains | RecA- hosts BLR(DE3) that minimize plasmid recombination | Stabilizing target plasmids |
| B834 Strain | A methionine auxotroph ideal for protein labeling applications | Protein labeling |
Ordering Information
Documentation
Related Products & Applications
Categories
| Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression |
| Application Guide | ||
| Expression Strains | Feature | Target Application |
| DE3 Hosts | Lysogens of bacteriophage λDE3 that express T7 RNA polymerase | Protein induction from T7 expression vectors |
| (DE3) pLysS Hosts | Express T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction | No protein/cell death due to toxic target protein |
| BL21 Strains | Deficient in Lon and OmpT proteases that minimize protein degradation | General protein expression |
| Origami™ 2 and Rosetta-gami™ Strains | TrxB/gor- hosts that minimize protein reduction in cytoplasm | Insoluble protein/no activity |
| Tuner™ and Rosetta-gami™ B Strains | LacY- hosts that attenuate expression/titrate IPTG and minimize protein misfolding | Insoluble protein/no activity |
| Rosetta and Rosetta-gami™ Strains | Supply rare tRNAs for improved full-length protein expression | Truncated protein |
| HMS174 and NovaBlue Strains | RecA- hosts BLR(DE3) that minimize plasmid recombination | Stabilizing target plasmids |
| B834 Strain | A methionine auxotroph ideal for protein labeling applications | Protein labeling |