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48-669MAG MILLIPLEX® Early Apoptosis Magnetic Bead 7-plex Kit - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate s

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
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48-669MAG
96-well plate  96-well plate
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      Overview

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Akt (pS473) BAD (Ser112) Bcl-2 (Ser70) p53 (Ser46) JNK (Thr183/Tyr185) Active Caspase-8 (Asp384) Active Caspase-9 (Asp315) HMplexLuminex xMAP
      Description
      Catalogue Number48-669MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX® Early Apoptosis Magnetic Bead 7-plex Kit - Cell Signaling Multiplex Assay
      OverviewApoptosis is an essential physiological process that plays a crucial role in normal development and homeostatic mechanisms of multicellular organisms. At least two major apoptotic pathways have been described in mammalian cells: the intrinsic pathway and the extrinsic pathway. Bcl-2 family members also play a pivotal role in the regulation of apoptosis as they reside upstream of irreversible cellular damage and focus much of their efforts at the level of mitochondria.

      The MILLIPLEX® 7-plex Early Apoptosis Magnetic Bead Kit (Cat. No. 48-669MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes:

      • Akt/PKB (Ser473)
      • BAD (Ser112)
      • Bcl-2 (Ser70)
      • Active Caspase-8
      • Active Caspase-9
      • JNK/SAPK1 (Thr183/Tyr185)
      • p53 (Ser46)
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Intra-Assay: < 15
      • Inter-Assay: < 20
      Panel TypeCell Signaling
      Quality LevelMQ200
      Applications
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate per well.
      • This kit must be run using Assay Buffer 2 (provided).
      • The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 μL/well at 40 to 1,000 µg/mL).
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • Akt (pS473)
      • BAD (Ser112)
      • Bcl-2 (Ser70)
      • p53 (Ser46)
      • JNK (Thr183/Tyr185)
      • Active Caspase-8 (Asp384)
      • Active Caspase-9 (Asp315)
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96-well plate
      Global Trade Item Number
      Catalogue Number GTIN
      48-669MAG 04053252820724