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EZHPI-15K Human Total Proinsulin ELISA

This Human Total Proinsulin ELISA is used to measure & quantify Proinsulin levels in Metabolism & Endocrine & Cell Signaling research.

Human Total Proinsulin ELISA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Fluorescent, Colorimetric Research Category: Metabolism Research Sub-Category: Obesity, Metabolic Disorders
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EZHPI-15K
96-well strip plate  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Proinsulin HELISAFluorescent Colorimetric
      Description
      Catalogue NumberEZHPI-15K
      DescriptionHuman Total Proinsulin ELISA
      Background InformationProinsulin, the precursor of insulin and C-peptide, is made by the beta cells of the pancreas. This kit measures the intact and intermediate metabolic forms of human proinsulin.
      References
      Product Information
      Detection methodFluorescent Colorimetric
      HS Code3002 15 90
      Precision, %
      • Inter-assay: 2.9–8.3
      • Intra-assay: 0.8–8.5
      Quality LevelMQ200
      Applications
      ApplicationThis Human Total Proinsulin ELISA is used to measure & quantify Proinsulin levels in Metabolism & Endocrine & Cell Signaling research.
      Key Applications
      • ELISA
      Application Notes20 μL sample volume 3 hour assay, room temperature
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • Proinsulin
      Entrez Gene Number
      Entrez Gene SummaryAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
      Gene Symbol
      • INS
      • proinsulin
      • insulin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
      SIZE: 110 amino acids; 11981 Da
      SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
      SUBCELLULAR LOCATION: Secreted.
      DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
      SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
      Accuracy
      • 88–103%
      Physicochemical Information
      Sensitivity
      • 0.5 pM
      Linearity of Dilution84–120%
      Standard Curve Range
      • 2–200 pM
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      EZHPI-15K 04053252320965