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71493 HRV 3C Protease

Highly efficient fusion tag removal at low temperature

HRV 3C Protease MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71493
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      		 Glass bottle 500 u
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      Description
      OverviewRecombinant type 14 3C protease from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. HRV 3C is a highly purified recombinant 6XHis-fusion protein, that recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln↓GlyPro. The small, 22-kDa size of the protease, optimal activity at 4°C, high specificity, and His•Tag® fusion make HRV 3C protease an ideal choice for rapid removal of fusion tags. The pET expression vectors pET-47b(+) through pET-50b(+) incorporate the HRV 3C protease cleavage site in combinations with His•Tag, S•Tag™, thioredoxin (Trx•Tag™), glutathione-S-transferase (GST•Tag™), and NusA (Nus•Tag™) sequences. The combination of pET-47b(+) to pET-50b(+) vectors for expression, HRV 3C protease for fusion tag cleavage, and Ni-NTA His•Bind® metal affinity chromatography for protein purification as well as protease and fusion tag removal allows production of recombinant proteins free of vector-encoded sequences. Other expression vectors, including the pET-52b(+) vector, the InsectDirect™ pIEx™-9 vector, and the multisystem pTriEx™-6 vector, all incorporate the HRV 3C protease cleavage site in combination with the Strep•Tag® II and His•Tag sequences. Supplied as: 1.8-3.0 U/µl in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.
      Catalogue Number71493
      Brand Family Novagen®
      References
      Product Information
      Activity1.8-3.0 U/µl
      Unit of DefinitionOne unit will cleave >95% of 100 µg His•Tag fusion control protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.
      •500 UHRV 3C Protease
      •10 µgHRV 3C Cleavage Control Protein
      •10 ml10X HRV 3C Cleavage Buffer
      FormulationIn 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.
      Quality LevelMQ400
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71493-3 07790788053277

      Documentation

      HRV 3C Protease Certificates of Analysis

      TitleLot Number
      71493

      Brochure

      Title
      Bulk Product Guide

      Citations

      Title
    • Yanqiu Yuan, et al. (2007) Crystal structure of a peptidoglycan glycosyltransferase suggests a model for processive glycan chain synthesis. Procedings of the National Academy of Science 104, 5348-5353.
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      User Protocols

      Title
      TB420