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391338 HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem

A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C. Absorbance (1.0 M, H2O, 260 nm):≤0.05.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid

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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
7365-45-9C₈H₁₈N₂O₄S

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              Description
              OverviewA zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
              Absorbance (1.0 M, H2O, 260 nm):≤0.05.
              Absorbance (1.0 M, H2O, 280 nm): ≤0.04
              Catalogue Number391338
              Brand Family Calbiochem®
              SynonymsN-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid
              References
              Product Information
              CAS number7365-45-9
              FormWhite crystalline powder
              Hill FormulaC₈H₁₈N₂O₄S
              Chemical formulaC₈H₁₈N₂O₄S
              PKA7.25 - 7.75 at 20°C
              Structure formula ImageStructure formula Image
              Quality LevelMQ100
              Applications
              Biological Information
              Purity≥99% by titration (dry basis)
              Physicochemical Information
              ContaminantsChloride: ≤0.01%; Sodium: ≤0.1%; Sulfate: ≤0.05%
              Heavy metals≤1 ppm
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSTL6809000
              Safety Information
              R PhraseR: 36/37/38

              Irritating to eyes, respiratory system and skin.
              S PhraseS: 26-37/39

              In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
              Wear suitable gloves and eye/face protection.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Irritant
              Storage +15°C to +30°C
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Global Trade Item Number
              Catalogue Number GTIN
              391338-100GM 07790788049805
              391338-25GM 04055977190168
              391338-500GM 07790788049829

              Documentation

              HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem SDS

              Title

              Safety Data Sheet (SDS) 

              HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem Certificates of Analysis

              TitleLot Number
              391338

              Brochure

              Title
              Bulk Product Guide
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision26-October-2009 RFH
              SynonymsN-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid
              DescriptionA zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.
              FormWhite crystalline powder
              CAS number7365-45-9
              RTECSTL6809000
              Chemical formulaC₈H₁₈N₂O₄S
              Structure formulaStructure formula
              Purity≥99% by titration (dry basis)
              ContaminantsChloride: ≤0.01%; Sodium: ≤0.1%; Sulfate: ≤0.05%
              SolubilityH₂O (1 M)
              Storage +15°C to +30°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.
              Toxicity Irritant
              Merck USA index14, 4654

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              Categories

              Life Science Research > Inhibitors and Biochemicals > Biochemicals > Buffers