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341635 Sigma-AldrichFibronectin, Human Plasma

Fibronectin, Human Plasma MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

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Overview

Replacement Information

Pricing & Availability

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Description
Overview	Native fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
Catalogue Number	341635
Brand Family	Calbiochem®

References
References	Akiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol. 59, 1. Krejci, K., and Fritz, H. 1976. FEBS Lett. 64, 152. Selected Citations Hoffmeister, M., et al. 2008. J. Biol. Chem. 283, 2297. Brown, S., et al. 2002. Nature 418, 200. Kim, M., FEBS Letters 581 1917.

Product Information
CAS number	86088-83-7
Form	Liquid
Formulation	In PBS, pH 7.3.
Quality Level	MQ200

Applications

Biological Information
Purity	single major band by SDS-PAGE
Source	Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information
Contaminants	none detected by IEP with antiserum to fibronectin or normal human serum

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Thaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
341635-5MG	04055977194920

Documentation

Fibronectin, Human Plasma SDS

Title
Safety Data Sheet (SDS)

Fibronectin, Human Plasma Certificates of Analysis

Title	Lot Number
341635	Enter Lot Number

References

Reference overview
Akiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol. 59, 1. Krejci, K., and Fritz, H. 1976. FEBS Lett. 64, 152. Selected Citations Hoffmeister, M., et al. 2008. J. Biol. Chem. 283, 2297. Brown, S., et al. 2002. Nature 418, 200. Kim, M., FEBS Letters 581 1917.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	01-July-2009 RFH
Description	Native fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
Form	Liquid
Formulation	In PBS, pH 7.3.
Concentration Label	Please refer to vial label for lot-specific concentration
Recommended reaction conditions	Protocol for Coating Tissue Culture Plates With Fibronectin Note: this protocol is meant to serve as a guideline. Optimization may be required for individual cell types. Note: This protocol is based on the use of 21 cm² dishes (Corning 25010, Costar 3060, Falcon 3002, or an equivalent 60 mm dish) and 1 mg fibronectin; 1 mg fibronectin is sufficient for coating 10 dishes at 5 µg/cm². 1. Thaw the fibronectin by placing the vial in a 37°C water bath and leaving it undisturbed until completely thawed. DO NOT DISTURB THE VIAL AT ANY TIME DURING THE THAWING PROCESS! If the vial is disturbed or removed prior to complete thawing, the fibronectin will form a gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing. 2. After thawing, bring the solution to a final volume of 20 ml with sterile, serum-free culture medium that has been pre-warmed to 37°C. This yields a fibronectin solution of 50 µg/ml. 3. Add 2 ml of this solution to each of 10 tissue culture dishes and swirl gently to completely coat the growth surface. 4. Allow the dishes to remain at room temperature for ~45 min to permit binding of the fibronectin to the surface of the dish. 5. Tilt each dish and remove the fibronectin using a sterile pipet. Do not permit the pipet tip to touch or disturb the growth surface. 6. Add the cell suspension in medium directly to the fibronectin-coated dish.
Source	Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number	86088-83-7
Purity	single major band by SDS-PAGE
Contaminants	none detected by IEP with antiserum to fibronectin or normal human serum
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Thaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
Merck USA index	14, 4075
References	Akiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol. 59, 1. Krejci, K., and Fritz, H. 1976. FEBS Lett. 64, 152. Selected Citations Hoffmeister, M., et al. 2008. J. Biol. Chem. 283, 2297. Brown, S., et al. 2002. Nature 418, 200. Kim, M., FEBS Letters 581 1917.

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