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ESG2207 ESGRO® Recombinant Rat LIF Protein

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ESG2207
10⁷ units  1 mL
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      Overview

      Key Spec Table

      Key ApplicationsSourcePurity
      CultrtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.The active component rat LIF (rtLIF) has been shown to be >85% pure by SDS-PAGE.
      Description
      Catalogue NumberESG2207
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionESGRO® Recombinant Rat LIF Protein
      OverviewApplications for Rat ESGRO® include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF.
      Alternate Names
      • Rat LIF
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified, sterile liquid. Rat ESGRO® is supplied in liquid form as 107 Units ESGRO® in 1.0 mL of 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl with 1% w/v bovine serum albumin BSA as a carrier for stability.

      Rat ESGRO® is supplied 0.22 micron sterile filtered, and tested negative for mycoplasma.
      Quality LevelMQ100
      Applications
      ApplicationESGRO Leukemia Inhibitory Factor (LIF) supplement for rat ES cell culture. Each vial contains 10^7 units/ml
      Key Applications
      • Stem Cell Culture
      Application NotesApplications for Rat ESGRO® include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama et al. 1998).

      Rat ES Cells: RtLIF produced from a transfected cell line, has been shown to effectively maintain the undifferentiated phenotype of rat ES cells when used at a concentration of 1000-1500 units / mL (Takahama et al. 1998).

      At this recommended concentration 106 units of ESGRO® is sufficient for 1L of tissue culture media.

      Visit www.esgro-lif.com for additional information
      Biological Information
      Concentration10 million units/1 mL
      PurityThe active component rat LIF (rtLIF) has been shown to be >85% pure by SDS-PAGE.
      SourcertLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.
      Specific ActivityRat ESGRO® is assessed both on murine ES {embryonic stem} cells (MBL-1) and on murine M1 myeloid leukemic cells. A standard of 50 Units is defined as the concentration of Rat ESGRO® in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies. 10<sup>6</sup> units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media. <br /> Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mLMurine myeloid leukemic, M1 Assay: Specific Activity > 10<sup>8</sup> units/mg. <br /><br /> Note: LIF should not be allowed to come in contact with ruminant or swine.
      Entrez Gene SummaryLeukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
      Gene Symbol
      • NM_022196
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
      SIZE: 202 amino acids; 22008 Da
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
      Media FormLiquid
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Product Usage Statements
      Quality AssuranceThe highest standards of quality control are used in the manufacture of this product. No warranty is provided that the sale or use of the product either alone, in combination with other products, or in the operation of any process, will not infringe patent, intellectual property or any other rights of third parties. This product is supplied for research purposes only. It is not for human therapeutic and/or diagnostic use.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRat ESGRO® is shipped on dry ice. This product is stable for at least 12 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells.

      For long term storage it is recommended that Rat ESGRO® concentrate be stored at -20°C.

      It is recommended that prior to use, Rat ESGRO® should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at -20°C. Freeze thawing will reduce potency and is not recommended.
      Packaging Information
      Material Size10⁷ units
      Material Package1 mL
      Global Trade Item Number
      Catalogue Number GTIN
      ESG2207 04053252320910