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AP180B Donkey Anti-Goat IgG Antibody, biotin-SP conjugate, Species Adsorbed

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AP180B
500 µL  
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      Overview

      Description
      Catalogue NumberAP180B
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDonkey Anti-Goat IgG Antibody, biotin-SP conjugate, Species Adsorbed
      Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
      Product Information
      FormatBiotin
      PresentationLyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.

      RECONSTITUTION:
      Reconstitute with 500 μL of sterile distilled water.
      Quality LevelMQ100
      Applications
      ApplicationDonkey anti-Goat IgG Antibody, biotin-SP conjugate, Species Adsorbed detects level of Donkey Goat IgG & has been published & validated for use in ELISA, FC, IF, IH & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry
      • Western Blotting
      Application NotesEIA: 1:20,000-1:400,000
      Western blots using enzyme-conjugated streptavidin: 1:20,000-1:400,000.
      Immunohistochemistry: 1:500-1:5,000
      Flow cytometry: 1:200-1:1,000
      Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000
      Optimal working dilutions must be determined by end user.
      Biological Information
      Concentration1 mg/mL
      HostDonkey
      SpecificitySpecific for Goat IgG.
      Species Reactivity
      • Goat
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoaffinity Purified and Immunodepletion
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size500 µL
      Global Trade Item Number
      Catalogue Number GTIN
      AP180B 04053252404924