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239803 Cyclopamine, V. californicum - CAS 4449-51-8 - Calbiochem

Cyclopamine, V. californicum, CAS 4449-51-8, is a cell-permeable steroidal alkaloid & cholesterol mimic that specifically antagonizes Sonic Hedgehog signaling through direct interaction with Smo.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Shh Signaling Antagonist I

Primary Target: Hh signaling in Shh-light2 assay
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239803
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
4449-51-8C₂₇H₄₁NO₂

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239803-1MG
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      Description
      OverviewA cell-permeable steroidal alkaloid and cholesterol mimic that displays both teratogenic and antitumor activities. It disrupts cholesterol bio-synthesis and specifically antagonizes shh (Sonic Hedgehog) signaling pathway through direct interaction with Smo (smoothened), a distant relative of G-protein-coupled receptors. A valuable tool for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control. Also available as a 10 mM solution in Ethanol (Cat. No. 239806).
      Catalogue Number239803
      Brand Family Calbiochem®
      SynonymsShh Signaling Antagonist I
      References
      ReferencesBerman, D.M., et al. 2003. Nature, 425, 846.
      Thayer, S.P., et al. 2003. Nature, 425, 851.
      Watkins, D.N., et al. 2003. Nature 422, 313.
      Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
      Berman, D.M., et al. 2002. Science 297, 1559.
      Taipale, J., et al. 2000. Nature 406, 1005.
      Kim, S.K., and Melton, D.A. 1998. Proc. Natl. Acad. Sci. USA 95, 13036.
      Cooper, M.K., et al. 1998. Science 280, 1603.
      Incardona, J.P., et al. 1998. Development 125, 3553.
      Product Information
      CAS number4449-51-8
      ATP CompetitiveN
      FormOff-white solid
      Hill FormulaC₂₇H₄₁NO₂
      Chemical formulaC₂₇H₄₁NO₂
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetHh signaling in Shh-light2 assay
      Primary Target IC<sub>50</sub>20 nM
      Purity≥97% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSGY0750000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Carcinogenic / Teratogenic
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      239803-1MG 04055977198867

      Documentation

      Cyclopamine, V. californicum - CAS 4449-51-8 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Cyclopamine, V. californicum - CAS 4449-51-8 - Calbiochem Certificates of Analysis

      TitleLot Number
      239803

      References

      Reference overview
      Berman, D.M., et al. 2003. Nature, 425, 846.
      Thayer, S.P., et al. 2003. Nature, 425, 851.
      Watkins, D.N., et al. 2003. Nature 422, 313.
      Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
      Berman, D.M., et al. 2002. Science 297, 1559.
      Taipale, J., et al. 2000. Nature 406, 1005.
      Kim, S.K., and Melton, D.A. 1998. Proc. Natl. Acad. Sci. USA 95, 13036.
      Cooper, M.K., et al. 1998. Science 280, 1603.
      Incardona, J.P., et al. 1998. Development 125, 3553.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 JSW
      SynonymsShh Signaling Antagonist I
      DescriptionA cell-permeable, steroidal alkaloid and cholesterol mimic that displays both teratogenic and anti-tumor properties. Disrupts cholesterol biosynthesis and specifically antagonizes shh (Sonic Hedgehog) signaling through direct interaction with Smo (smoothened), a protein that is related to G-protein-coupled receptors. Useful for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control.
      FormOff-white solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number4449-51-8
      RTECSGY0750000
      Chemical formulaC₂₇H₄₁NO₂
      Structure formulaStructure formula
      Purity≥97% by HPLC
      SolubilityDMSO (4 mg/ml), Ethanol (5 mg/ml), or DMF (10 mg/ml). Ethanol stock solutions may require warming to achieve complete solubilization.
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Carcinogenic / Teratogenic
      ReferencesBerman, D.M., et al. 2003. Nature, 425, 846.
      Thayer, S.P., et al. 2003. Nature, 425, 851.
      Watkins, D.N., et al. 2003. Nature 422, 313.
      Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
      Berman, D.M., et al. 2002. Science 297, 1559.
      Taipale, J., et al. 2000. Nature 406, 1005.
      Kim, S.K., and Melton, D.A. 1998. Proc. Natl. Acad. Sci. USA 95, 13036.
      Cooper, M.K., et al. 1998. Science 280, 1603.
      Incardona, J.P., et al. 1998. Development 125, 3553.