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233153 Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem

A protective agent for SH groups Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Dithiothreitol, DTT

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233153
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
3483-12-3C₄H₁₀O₂S₂

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      Description
      OverviewA protective agent for SH groups.
      Absorbance: (100 mM, 280 nm):≤0.1. Oxidized dithiothreitol:≤0.5%.
      Catalogue Number233153
      Brand Family Calbiochem®
      SynonymsDithiothreitol, DTT
      References
      ReferencesChau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
      Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
      Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
      Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
      Shaked, Z., et al. 1980. Biochemistry 19, 4156.
      Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
      Cleland, W.W. 1964. Biochemistry 3, 480.
      Product Information
      CAS number3483-12-3
      FormWhite solid
      Hill FormulaC₄H₁₀O₂S₂
      Chemical formulaC₄H₁₀O₂S₂
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥99% by titration
      Physicochemical Information
      ContaminantsHeavy metals: <1 ppm
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSEK1610000
      Safety Information
      R PhraseR: 22-36/38

      Harmful if swallowed.
      Irritating to eyes and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      233153-1GM 04055977217643

      Documentation

      Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem Certificates of Analysis

      TitleLot Number
      233153

      References

      Reference overview
      Chau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
      Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
      Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
      Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
      Shaked, Z., et al. 1980. Biochemistry 19, 4156.
      Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
      Cleland, W.W. 1964. Biochemistry 3, 480.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2018 JSW
      SynonymsDithiothreitol, DTT
      DescriptionDTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes, protecting protein sulfhydryls and restoring enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL™ Reagent, Cat. No. 233157) can be used. Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.
      FormWhite solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number3483-12-3
      RTECSEK1610000
      Chemical formulaC₄H₁₀O₂S₂
      Purity≥99% by titration
      ContaminantsHeavy metals: <1 ppm
      SolubilityEthanol or H₂O (200 mM)
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Harmful
      Merck USA index14, 3376
      ReferencesChau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
      Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
      Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
      Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
      Shaked, Z., et al. 1980. Biochemistry 19, 4156.
      Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
      Cleland, W.W. 1964. Biochemistry 3, 480.