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627610 Caspase Inhibitor I - CAS 187389-52-2 - Calbiochem

Z-VAD-FMK, CAS 187389-52-2, is a cell-permeable, irreversible, pan-caspase inhibitor.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Z-VAD (OMe)-FMK

Primary Target: Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells
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627610
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
187389-52-2C₂₂H₃₀FN₃O₇

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627610-1MG
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      Description
      OverviewA cell-permeable, irreversible, pan-caspase inhibitor. Inhibits Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells. Shown to enhance the freeze-thaw survival of human embryonic stem cells. When using with purified native or recombinant enzyme, pre-treatment with an esterase is required.
      Catalogue Number627610
      Brand Family Calbiochem®
      SynonymsZ-VAD (OMe)-FMK
      References
      ReferencesHeng, B.C., et al. 2007. Biosci. Rep. 27, 257.
      Martin, U., et al. 2007. Apoptosis 12, 525.
      Misaghi, S., et al. 2004. Chem. Biol. 11, 1677.
      Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
      McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
      Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
      Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
      An, S. and Know, K.A. 1996. FEBS Lett. 386, 115.
      Cain, K., et al. 1996. Biochem J. 314, 27.
      Mirzoeva, O.K., et al. 1996. FEBS Lett. 396, 266.
      Pronk, G.J., et al. 1996. Science 271, 808.
      Slee, E.A., et al. 1996. Biochem. J. 315, 21.
      Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
      Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
      Zhu, H., et al. 1995. FEBS Lett. 374, 303.
      Product Information
      CAS number187389-52-2
      ATP CompetitiveN
      FormWhite solid
      Hill FormulaC₂₂H₃₀FN₃O₇
      Chemical formulaC₂₂H₃₀FN₃O₇
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetFas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells
      PuritySingle spot by TLC
      Physicochemical Information
      Cell permeableY
      Peptide SequenceZ-Val-Ala-Asp(OMe)-CH₂F*
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      627610-1MG 04055977262810

      Documentation

      Caspase Inhibitor I - CAS 187389-52-2 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Caspase Inhibitor I - CAS 187389-52-2 - Calbiochem Certificates of Analysis

      TitleLot Number
      627610

      References

      Reference overview
      Heng, B.C., et al. 2007. Biosci. Rep. 27, 257.
      Martin, U., et al. 2007. Apoptosis 12, 525.
      Misaghi, S., et al. 2004. Chem. Biol. 11, 1677.
      Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
      McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
      Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
      Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
      An, S. and Know, K.A. 1996. FEBS Lett. 386, 115.
      Cain, K., et al. 1996. Biochem J. 314, 27.
      Mirzoeva, O.K., et al. 1996. FEBS Lett. 396, 266.
      Pronk, G.J., et al. 1996. Science 271, 808.
      Slee, E.A., et al. 1996. Biochem. J. 315, 21.
      Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
      Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
      Zhu, H., et al. 1995. FEBS Lett. 374, 303.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure

      Citations

      Title
    • Heng, B.C., et al. 2007. Biosci. Rep. 27, 257.
    • Naohide Watanabe and Eric Lam. 2005. Journal of Biological Chemistry 280, 14691.
    • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-February-2012 RFH
      SynonymsZ-VAD (OMe)-FMK
      DescriptionA cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.
      FormWhite solid
      CAS number187389-52-2
      Chemical formulaC₂₂H₃₀FN₃O₇
      Peptide SequenceZ-Val-Ala-Asp(OMe)-CH₂F*
      Structure formulaStructure formula
      PuritySingle spot by TLC
      SolubilityDMSO (50 mM)
      Storage Protect from moisture
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesHeng, B.C., et al. 2007. Biosci. Rep. 27, 257.
      Martin, U., et al. 2007. Apoptosis 12, 525.
      Misaghi, S., et al. 2004. Chem. Biol. 11, 1677.
      Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
      McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
      Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
      Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
      An, S. and Know, K.A. 1996. FEBS Lett. 386, 115.
      Cain, K., et al. 1996. Biochem J. 314, 27.
      Mirzoeva, O.K., et al. 1996. FEBS Lett. 396, 266.
      Pronk, G.J., et al. 1996. Science 271, 808.
      Slee, E.A., et al. 1996. Biochem. J. 315, 21.
      Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
      Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
      Zhu, H., et al. 1995. FEBS Lett. 374, 303.
      Citation
    • Heng, B.C., et al. 2007. Biosci. Rep. 27, 257.
    • Naohide Watanabe and Eric Lam. 2005. Journal of Biological Chemistry 280, 14691.
    • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
      		•