Millipore Sigma Vibrant Logo

391334 BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid

View Products on Sigmaaldrich.com
391334
View Pricing & Availability

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
10191-18-1C₆H₁₅NO₅S

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
391334-100GM
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Glass bottle 100 gm
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      OverviewBES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
      Absorbance (100 mM, 260 nm):≤0.05.
      Catalogue Number391334
      Brand Family Calbiochem®
      SynonymsN,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
      References
      ReferencesDe Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
      Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.
      Product Information
      CAS number10191-18-1
      FormWhite solid
      Hill FormulaC₆H₁₅NO₅S
      Chemical formulaC₆H₁₅NO₅S
      PKA7.12 at 20°C
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥99% by titration (dry basis)
      Physicochemical Information
      Heavy metals≤1 ppm
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSKI7363500
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      391334-100GM 07790788058364

      Documentation

      BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem Certificates of Analysis

      TitleLot Number
      391334

      References

      Reference overview
      De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
      Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-July-2008 RFH
      SynonymsN,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
      DescriptionBES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.
      FormWhite solid
      CAS number10191-18-1
      RTECSKI7363500
      Chemical formulaC₆H₁₅NO₅S
      Purity≥99% by titration (dry basis)
      SolubilityH₂O (1 mg/ml)
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
      Toxicity Standard Handling
      ReferencesDe Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
      Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.