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16-234 Anti-FAK Antibody, clone 4.47, Alexa Fluor® 555 conjugate

Anti-FAK Antibody, clone 4.47, Alexa Fluor 555 conjugate detects level of FAK & has been published & validated for use in FC, WB & IC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: PTK2, pp125FAK, FADK, FAK1, FAK UniProt Number: Q05397
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16-234
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, WB, ICCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue Number16-234
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-FAK Antibody, clone 4.47, Alexa Fluor® 555 conjugate
      References
      Product Information
      FormatAlexaFluor®555
      Quality LevelMQ100
      Applications
      ApplicationAnti-FAK Antibody, clone 4.47, Alexa Fluor 555 conjugate detects level of FAK & has been published & validated for use in FC, WB & IC.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 1-423 of human FAK (focal adhesion kinase)
      Cloneclone 4.47
      HostMouse
      Specificityp125FAK, does not cross react with Pyk-2
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
      Gene Symbol
      • PTK2
      • pp125FAK
      • FADK
      • FAK1
      • FAK
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
      SIZE: 1052 amino acids; 119233 Da
      SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
      SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
      TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
      DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
      PTM: Phosphorylated on 6 tyrosine residues upon activation.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
      Molecular Weight125kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunostaining in 3T3 cells
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 4°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      16-234 04053252470844

      Documentation

      Anti-FAK Antibody, clone 4.47, Alexa Fluor® 555 conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-FAK Antibody, clone 4.47, Alexa Fluor® 555 conjugate Certificates of Analysis

      TitleLot Number
      Anti-FAK, clone 4.47, Alexa Fluor#174; 555 conjugate - DAM1432877 DAM1432877
      Anti-FAK, clone 4.47, Alexa Fluorreg; 555 conjugate - 30488 30488
      Anti-FAK, clone 4.47, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1) 3074352
      Anti-FAK, clone 4.47, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1) 2900701
      Anti-FAK, clone 4.47, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1) - 2251743 2251743
      Anti-FAK, clone 4.47, Alexa Fluor® 555 conjugate - 3429411 3429411
      Anti-FAK, clone 4.47, Alexa Fluor® 555 conjugate - 3823361 3823361
      Anti-FAK, clone 4.47, Alexa Fluor® 555 conjugate - 4107775 4107775
      Anti-FAK, clone 4.47, Alexa Fluor® 555 conjugate - 4133053 4133053
      Anti-FAK, clone 4.47, Alexa Fluor® 555 conjugate -2616232 2616232
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      References

      Reference overviewPub Med ID
      Advances in high content screening for drug discovery.
      Giuliano, Kenneth A, et al.
      Assay and drug development technologies, 1: 565-77 (2003)  2003

      		15090253 15090253

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies