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70781 pET Expression System 30 - Novagen

pET vectors plus host strains with induction control

pET Expression System 30 - Novagen: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
70781
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      Description
      OverviewpET Expression Systems and pET
      pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

      Components: pET Expression Systems
      Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
      • 10 µg pET vector DNA (for each indicated plasmid)
      • 0.2 ml BL21 Glycerol Stock
      • 0.2 ml BL21(DE3) Glycerol Stock
      • 0.2 ml BL21(DE3)pLysS Glycerol Stock
      • 0.2 ml Induction Control Glycerol Stock

      Components: pET Expression Systems plus Competent Cells
      pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
      • 0.2 ml NovaBlue Competent Cells
      • 0.2 ml BL21(DE3) Competent Cells
      • 0.2 ml BL21(DE3)pLysS Competent Cells
      • 2 × 0.2 ml SOC Medium
      • 10 µl Test Plasmid

      These components are sufficient for up to 10 transformations in each host.

      Purification and Detection Reagents
      Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

      pET Expression System 3
      The pET Expression System 3 contains 10 µg each of the four versions of pET-30 (pET-30a–c(+)). The pET-30a–c(+) vectors carry an N-terminal His•Tag®/thrombin/S•Tag™/enterokinase configuration plus an optional C-terminal His•Tag sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB095VM.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number70781
      Brand Family Novagen®
      References
      Product Information
      •10 µg eachpET-30a-c(+) vector DNA
      •0.2 mlHost bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
      •0.2 mlInduction control clone, glycerol stock
      Fusion tagHis•Tag, S•Tag
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      70781-3 04055977273212

      Documentation

      pET Expression System 30 - Novagen Certificati d'Analisi

      TitoloNumero di lotto
      70781

      Citazioni

      Titolo
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    • Kai-Hong Zhao, et al. (2006) Chromophore attachment to phycobiliprotein -subunits: phycocyanobilin:cysteine-84 phycobiliprotein lyase activity of Cpes-like protein from Anabaena SP. PCC7120. Journal of Biological Chemistry 281, 8573-8581.
    • Carmen Brenner, et al. (2005) Myc represses transcription through recruitment of DNA methyltransferase corepressor. European Molecular Biology Organization Journal 24, 336-346.
    • Min-Hsuan Chen, et al. (2005) Phospholipid scramblase 1 (PLSCR1) contains a non-classical nuclear localization signal with unique binding site in importin a. Journal of Biological Chemistry 280, 10599-10606.
    • Erika Crouch, et al. (2005) Ligand specificity of human surfactant protein D: expression of a mutant trimeris collection that shows enhanced interactions with influenza A virus. Journal of Biological Chemistry 280, 17046-17056.
    • Neelam Desai, et al. (2005) Two steps in Maf1 dependent repression of transcription by RNA Polymerase III. Journal of Biological Chemistry 280, 6455-6462.
    • Sanghamitra Dey, Gregory A. Grant and James C. Sacchettini. (2005) Crystal structure of mycobacterium tuberculosis D-3-phosphoglycerate dehydrogenase: extreme asymmetry in a tetramer of identical subunits. Journal of Biological Chemistry 280, 14892-14899.
    • Akari Fukuda, et al. (2005) Differential distribution of proteins expressed in companion cells in the sieve element-companion cell complex of rice plants. Plant and Cell Physiology 46, 1779-1786.
    • Jong-Min Han, et al. (2005) Structural and functional properties of V156K and A158E mutants of apolipoprotein A-I in the lipid-free and lipid-bound state. Journal of Lipid Research 46, 589-596.
    • Patricia L. Herman, et al. (2005) A three component dicamba O-demethylase from Pseudomonas maltophilia, strain DI-6: Gene isolation, characterization, and heterologous expression. Journal of Biological Chemistry 280, 24759-24767.
    • Bruce J. Herron, et al. (2005) A mutation in NFκB interacting protein 1 results in cardiomyopathy and abnormal skin development in wa3 mice. Human Molecular Genetics 14, 667-677.
    • Antonia H. Holway, Crystal Hung and W. Matthew Michael. (2005) Systematic, RNAi-mediated identification of mus-101 modifier genes in Caenorhabditis elegans. Genetics 169, 1451-1460.
    • J. K. Jang, T. Rahman and K. S. McKim. (2005) The kinesinlike protein subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. 16, 4684-4694.
    • Aki Jarvinen, et al. (2005) Metabolic stability of alpha -methylated polyamine derivatives and their use as substitutes for the natural polyamines. Journal of Biological Chemistry 280, 6595-6601.
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    • Margarita L. Malakhova, et al. (2005) Point mutational analysis of the liganding site in human glycolipid transfer protein: functionally of the complex. Journal of Biological Chemistry 280, 26312-26320.
    • Victoria L. Marsh, Sew Yeu Peak-Chew and Stephen D. Bell. (2005) Sir2 and the acetyltransferase, Pat, regulate the archael chromatin protein, Alba. Journal of Biological Chemistry 280, 21122-21128.
    • Satoru Moritoh, et al. (2005) RNAi-mediated silencing of OsGEN-L (OsGEN-like), a new member of the RAD2/XPG nuclease family, causes male sterility by defect of microspore development in rice. Plant and Cell Physiology 46, 699-715.
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    • Kevin Pearson, et al. (2005) Specific sequences in the N-and C-termini of apolipoprotein A-IV modulate its conformation and lipid association. Journal of Biological Chemistry 280, 38576-38582.
    • Daniel Picard, C. Cheng Kao and Katalin A. Hudak. (2005) Pokeweed antiviral protein inhibits brome mosaic virus replication in plant cells. Journal of Biological Chemistry 280, 20069-20075.
    • Alexander J. Ruthenburg, et al. (2005) A superhelical spiral in the Escherichia coli DNA gyrase A C-terminal domain imparts unidirectional supercoiling bias. Journal of Biological Chemistry 280, 26177-26184.
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    • Masako Shimada, et al. (2005) The receptor for parathyroid hormone and parathyroid hormone-related peptide is hydrolyzed and its signaling properties are altered by directly binding the calpain small subunit. Endocrinology 146, 2336-2344.
    • David Stuckler, et al. (2005)

      Protocolli per l'uso

      Titolo
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      Mappa di un vettore

      Titolo
      TB095 pET-30a-c(+) Vector Map

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      Categorie

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors