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SCC241 YUMM5.4 Mouse Melanoma Cell Line

The YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM5.4 retains WT Pten, permitting comparative analysis for parsing distinct contributions of PI3K-Akt signaling in melanoma tumorigenesis and immune evasion.

NovitàSCC241
>1x10^6 cells.vial  
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      Replacement Information
      Description
      Catalogue NumberSCC241
      DescriptionYUMM5.4 Mouse Melanoma Cell Line
      OverviewThe YUMM (Yale University Mouse Melanoma) cell lines were developed as models that recapitulate genetic drivers found in a significant proportion of human melanomas. YUMM5.4 cells are syngeneic with the immunocompetent C57Bl/6 mouse model.
      Alternate Names
      • Yale University Mouse Melanoma cell line
      • Yale University Mouse Melanoma 5.4 cell line
      Background InformationImmunocompetent genetically-engineered mouse models with select genetic mutations that drive melanoma are fundamental for studying candidate immunotherapies, but are limited by the need to maintain colonies of multiple genotypes necessary to generate models with appropriate genetic backgrounds.​
      Approximately half of all melanomas harbor Braf mutations, of which the V600E mutation comprises 90% of cases, while the tumor suppressor p53 is inactivated in 90% of melanomas. The YUMM5.4 cell line harbors the activating Braf V600E mutation and is negative for wild-type p53.


      Source
      YUMM5.4 mouse melanoma cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically engineered mouse model were introduced by backcrossing.


      References:
      1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.
      2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.
      References
      Product Information
      Components
      • ≥1x106 viable YUMM5.4 Cells: (Catalog No. SCC241). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationThe YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM5.4 retains WT Pten, permitting comparative analysis for parsing distinct contributions of PI3K-Akt signaling in melanoma tumorigenesis and immune evasion.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
      Biological Information
      HostMouse
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance"• Each vial contains ≥ 1X10⁶ viable cells.
      • YUMM5.4 cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for interspecies contamination from rat, chinese hamster, Golden Syrian hamster, human and nonhuman primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsYUMM5.4 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size>1x10^6 cells.vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      SCC241 04065270885050