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SCC240 YUMM5.2 Mouse Melanoma Cell Line

The YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM5.2 cells are syngenic with C57BL/6 and retain markers of the Braf/Pten mouse model.

SCC240
≥1X10⁶ cells/vial   
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      Replacement Information
      Description
      Catalogue NumberSCC240
      DescriptionYUMM5.2 Mouse Melanoma Cell Line
      OverviewSource
      The YUMM5.2 mouse melanoma cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a female C57BL/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing. The YUMM5.2 cell line harbors the Braf V600E mutation and is homozygous negative for Cdkn2.1​
      Alternate Names
      • Yale University Mouse Melanoma cell line
      • Yale University Mouse Melanoma 5.2 cell line
      Background InformationThe promise of immune-based therapies for cancer diagnoses and increasingly successful application of these approaches have emphasized the need for immunocompetent models for examination of immunological responses to cancer cells. Immunocompetent genetically-engineered mouse models with distinct genetic drivers of melanoma are essential for identifying potential immunotherapies, but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgrounds.

      YUMM5.2 cells are syngenic with the immunocompetent C57BL/6 mouse background and maintain genetic markers of the Braf/Pten mouse model. Approximately half of all melanomas contain Braf mutations, of which the V600E mutation comprises 90% of cases, while the tumor suppressor p53 is inactivated in 90% of melanomas. The YUMM5.2 cell line harbors the activating Braf V600E mutation and is negative for p53. The YUMM5.2 mouse melanoma cell line represents a valuable tool for investigating tumor biology and developing therapeutic approaches for melanomas with this highly prevalent mutational profile. ​

      Mutation: BrafV600E/wt p53−/−

      References:
      1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.
      2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationThe YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM5.2 cells are syngenic with C57BL/6 and retain markers of the Braf/Pten mouse model.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      SCC240 04065269371212