Millipore Sigma Vibrant Logo

70954 Rosetta™(DE3) Competent Cells - Novagen

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Rosetta™(DE3) Competent Cells - Novagen: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
View Products on Sigmaaldrich.com
70954
Visualizza prezzi e disponibilità

Panoramica

Replacement Information

Prezzi e disponibilità

Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
70954-3
Verifica della disponibilità in corso...
—
Disponibilità limitata Disponibilità limitata
A magazzino 
Fuori produzione
Disponibili quantità limitate
La disponibilità deve essere confermata
    Prodotti rimanenti: riceverà un nostro avviso
      Prodotti rimanenti: riceverà un nostro avviso
      Will advise
      Contatti il Servizio Clienti
      Contact Customer Service

      		 Fiala di plastica 0.4 ml
      Ricerca del prezzo in corso...
      Non è stato possibile trovare il prezzo
      La quantità minima deve essere un multiplo di
      Maximum Quantity is
      Al termine dell'ordine Maggiori informazioni
      Lei ha salvato ()
       
      —
      Richiedi il prezzo
      		 —
      70954-4
      Verifica della disponibilità in corso...
      —
      Disponibilità limitata Disponibilità limitata
      A magazzino 
      Fuori produzione
      Disponibili quantità limitate
      La disponibilità deve essere confermata
        Prodotti rimanenti: riceverà un nostro avviso
          Prodotti rimanenti: riceverà un nostro avviso
          Will advise
          Contatti il Servizio Clienti
          Contact Customer Service

          		 Fiala di plastica 1 ml
          Ricerca del prezzo in corso...
          Non è stato possibile trovare il prezzo
          La quantità minima deve essere un multiplo di
          Maximum Quantity is
          Al termine dell'ordine Maggiori informazioni
          Lei ha salvato ()
           
          —
          Richiedi il prezzo
          		 —
          Description
          OverviewRosetta™ host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters. In Rosetta(DE3)pLysS, the rare tRNA genes are present on the same plasmids that carries the T7 lysozyme gene.

          DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
          Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE (CamR)


          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number70954
          Brand Family Novagen®
          References
          Product Information
          Components
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Numero di catalogo GTIN
          70954-3 07790788052904
          70954-4 07790788052911

          Documentation

          Rosetta™(DE3) Competent Cells - Novagen MSDS

          Titolo

          Scheda di sicurezza (MSDS) 

          Rosetta™(DE3) Competent Cells - Novagen Certificati d'Analisi

          TitoloNumero di lotto
          70954

          Brochure

          Titolo
          Competent Cell Brochure

          Citazioni

          Titolo
        • Graeme S. Garvey, Susan P. McCormick and Ivan Rayment. (2008) Structural and functional characterization of the TRI101 trichothecene 3-O-acetyltransferase from Fusarium sporotrichioides and Fusarium graminearum: kinetic insights to combating Fusarium head blight. Journal of Biological Chemistry 283, 1660-1669.
        • Maximilian J. Hartl, et al. (2008) AZT resistance of simian foamy virus reverse transcriptase is based on the excision of AZTMP in the presence of ATP. Nucleic Acids Research 36, 1009-1016.
        • Lucia Banci, et al. (2007) The different intermolecular interactions of the soluble copper-binding domains of the Menkes protein, ATP7A. Journal of Biological Chemistry 282, 23140-23146.
        • Priscilla Braglia, et al. (2007) Requirement of Nhp6 proteins for transcription of a subset of tRNA genes and heterochromatin barrier function in Saccharomyces cerevisiae. Molecular and Cellular Biology 27, 1545-1557.
        • Marina Brucet, et al. (2007) Structure of the dimeric exonuclease TREX1 in complex with DNA displays a proline-rich binding site for WW domains. Journal of Biological Chemistry 282, 14547-14557.
        • Hong Wei Chu, et al. (2007) Function and regulation of SPLUNC1 protein in Mycoplasma infection and allergic inflammation. Journal of Immunology 179, 3995-4002.
        • L'ubomira Cubonova, et al. (2007) Spontaneous trpY mutants and mutational analysis of the TrpY archaeal transcription regulator. Journal of Bacteriology 189, 4338-4342.
        • Marcel Hohl, et al. (2007) Domain swapping between FEN-1 and XPG defines regions in XPG that mediate nucleotide excision repair activity and substrate specificity. Nucleic Acids Research 35, 3053-3063.
        • Anette Thysse Jonstrup, et al. (2007) The 1.4-Å crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme. Nucleic Acids Research 35, 3153-3164.
        • Sebastian Kalamajski, Anders Aspberg and Ake Oldberg. (2007) The decorin sequence SYIRIADTNIT binds collagen type I. Journal of Biological Chemistry 282, 16062-16067.
        • Lilia Kaustov, et al. (2007) The conserved CPH domains of Cul7 and PARC are protein-protein interaction modules that bind the tetramerization domain of P53. Journal of Biological Chemistry 282, 11300-11307.
        • Changkeun Lee, et al. (2007) Yeast mitochondrial initiator tRNA is methylated at G37 by the TRM5-encoded tRNA (Guanine-N1-)-methyltransferase. Journal of Biological Chemistry 282, 27744-27753.
        • Veli-Matti Leppänen, et al. (2007) Crystal structure of the N-terminal NC4 domain of collagen IX, a zinc binding member of the laminin-neurexin-sex hormone binding globulin (LNS) domain family. Journal of Biological Chemistry 282, 23219-23230.
        • Yoshihiro Nakamura, et al. (2007) Crystal structure of the human BRD2 bromodomain: insights into dimerization and recognition of acetylated histone H4. journal of Biological Chemistry 282, 4193-4201.
        • Pascale Plamondon, Nicole R. Luke and Anthony A. Campagnari. (2007) Identification of a novel two-partner secretion locus in Moraxella catarrhalis. Infection and Immunity 75, 2929-2936.
        • Biagio Pucci, et al. (2007) Modular organization of the Sulfolobus solfataricus mini-chromosome maintenance protein. Journal of Biological Chemistry 282, 12574-12582.
        • Giuseppe D. Tocchini-Valentini, Paolo Fruscoloni and Glauco P. Tocchini-Valentini. (2007) The dawn of dominance by the mature domain in tRNA splicing. Procedings of the National Academy of Science 104, 12300-12305.
        • Marielle E. Yohe, et al. (2007) Auto-inhibition of the Dbl family protein Tim by an N-terminal helical motif. Journal of Biological Chemistry 282, 13813-13823.
        • Zubair M. Ahmed, et al. (2006) The tip-link antigen, a protein associated with the transduction complex of sensory hair cells, is protocadherin-15. Journal of Neuroscience 26, 7022-7034.
        • Kenji Kashiwagi, et al. (2006) Frame shuffling: a novel method for in vitro protein evolution. Protein Engineering Design and Selection 19, 135-140.
        • Amparo Lima, et al. (2006) A redox-active FKBP-type immunophilin functions in accumulation of the photosystem II supercomplex in Arabidopsis thaliana. Procedings of the National Academy of Science 103, 12631-12636.
        • Chad E. Metcalf and David A. Wassarman. (2006) DNA binding properties of TAF1 isoforms with two AT-hooks. Journal of Biological Chemistry 281, 30015-30023.
        • James Schelert, et al. (2006) Regulation of mercury resistance in the crenarchaeote Sulfolobus solfataricus. Journal of Bacteriology 188, 7141-7150.
        • Yi-Hsien Su and Victor D. Vacquier. (2006) Cyclic GMP-specific phosphodiesterase-5 regulates motility of sea urchin spermatozoa. Molecular Biology of the Cell 17, 114-121.
        • Guido Veit, et al. (2006) Collagen XXVIII, a novel von Willebrand Factor A domain-containing protein with many imperfections in the collagenous domain. Journal of Biological Chemistry 281, 3494-3504.
        • Jerome Vicogne, et al. (2006) Asymmetric phospholipid distribution drives in vitro reconstituted SNARE-dependent membrane fusion. Procedings of the National Academy of Science 103, 14761-14766.
        • Hao Xu and William Wickner. (2006) BEM1P is a positive regulator of the homotypic fusion of yeast vacuoles. Journal of Biological Chemistry 281, 27158-27166.
        • Philipp Beinker, et al. (2005) Biochemical coupling of the two nucleotide binding domains of ClpB: Covalent linkage is not a prerequisite for chaperone activity. Journal of Biological Chemistry 280, 37965-37973.
        • Eric R. Bonner, et al. (2005) Molecular basis of cysteine biosynthesis in plants: structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana. Journal of Biological Chemistry 280, 38803-38813.
        • Robert Court, et al. (2005) How the human telomeric proteins TRF1 and TRF2 recognize telomeric DNA: a view from high-resolution crystal structures. European Molecular Biology Organization Reports 6, 39-45.
        • Sonja A. Dames, et al. (2005) The solution structure of the FATC domain of the protein kinase target of rapamycin suggests a role for redox-dependent structural and cellular stability. Journal of Biological Chemistry 280, 20558-20564.
        • Thomas T. Egelhoff, Daniel Croft and Paul A. Steimle. (2005) Actin activation of myosin heavy chain kinase A in Dictyostelium: a biochemical mechanism for the spatial regultion of myosin II filament disassembly. Journal of Biological Chemistry 280, 2879-2887.
        • Pierre Fotso, et al. (2005) Cog1p plays a central role in the organization of the yeast conserved oligomeric golgi (COG) complex. Journal of Biological Chemistry 280, 27613-27623.
        • Johannes Haugstetter, Thomas Blicher and Lars Ellgaard. (2005) Identification and characterization of a novel thioredoxin-related transmembrane protein of the endoplasmic reticulum. Journal of Biological Chemistry 280, 8371-8380.
        • Meike Hoffmeister, et al. (2005) Mitochondrial trans-2-enoyl-coA reductase of wax ester fermentation from Euglena gracilis defines a new family of enzymes involved in lipid synthesis. Journal of Biological Chemistry 280, 4329-4338.
        • Shinpei Katou, et al. (2005) Catalytic activation of plant MAPK phosphatase NtMKP1 by its physiological substrate SIPK, but not by calmodulins. Journal of Biological Chemistry 280, 39569-39581.
        • Christopher K. Kennaway, et al. (2005) Dodecameric structure of the small heat shock protein Acr1 from Mycobacterium tuberculosis. Journal of Biological Chemistry 280, 33419-33425.
        • Beth T. Vander Kooi, et al. (2005) The glucose-6-phosphatase catalytic subunit gene promoter contains both positive and negative glucocorticoid response elements. Molecular Endocrinology 19, 3001-3022.
        • Michael Kothe, et al. (2005) Direct demonstration of carbamoyl phosphate formation on the C-terminal domain of carbamoyl phosphate synthetase. Protein Science 14, 37-44.
        • Sabbi Lall, Fabio Piano and Richard E. Davis. (2005) Caenorhabditis elegans decapping proteins: localization and functional analysis of Dcp1, Dcp2, and DcpS during embryogenesis. Molecular Biology of the Cell 16, 5880-5890.
        • Peter A. Lemaire, Jeffrey Lary and James L. Cole. (2005) Mechanism of PKR activation: dimerization and kinase activation in the absence of double-stranded RNA. Journal of Molecular Biology 345, 81-90.
        • Roberta Leonardi, et al. (2005) A pantothenate kinase from Staphylococcus aureus refractory to feedback regulation by coenzyme A. Journal of Biological Chemistry
          		•

          Protocolli per l'uso

          Titolo
          TB009 Competent Cells
          TB053 Academic and Non-profit Laboratory Assurance Letter

          Prodotti e applicazioni correlate

          Related Products

          Numero di catalogo Descrizione  
          70953 Rosetta™ Competent Cells - Novagen Prezzi e disponibilità
          70956 Rosetta™(DE3)pLysS Competent Cells - Novagen Prezzi e disponibilità

          Prodotti correlati per: Brand Facete

          Rosetta™

          Categorie

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression