Millipore Sigma Vibrant Logo

539125 Protease Assay Kit

View Products on Sigmaaldrich.com
539125
Visualizza prezzi e disponibilità

Panoramica

Replacement Information

Tabella delle specifiche principali

Detection Methods
Colorimetric

Prezzi e disponibilità

Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
539125-1KIT
Verifica della disponibilità in corso...
Disponibilità limitata
Disponibilità limitata
A magazzino 
Fuori produzione
Disponibili quantità limitate
La disponibilità deve essere confermata
    Prodotti rimanenti: riceverà un nostro avviso
      Prodotti rimanenti: riceverà un nostro avviso
      Will advise
      Contatti il Servizio Clienti
      Contact Customer Service

      Bottiglia di vetro 1 kit
      Ricerca del prezzo in corso...
      Non è stato possibile trovare il prezzo
      La quantità minima deve essere un multiplo di
      Maximum Quantity is
      Al termine dell'ordine Maggiori informazioni
      Lei ha salvato ()
       
      Richiedi il prezzo
      Description
      OverviewA kit suitable for quantitative array of a wide variety of proteases in biological samples, utilizing FTC-casein as a substrate. Enzyme activities that have been detected with this assay kit include: chymotrypsin, elastase, plasminogen, PRONASE® Protease, subtilisin, thermolysin, and trypsin.
      Catalogue Number539125
      Brand Family Calbiochem®
      Materials Required but Not Delivered Trichloroacetic acid (TCA), 5%
      Spectrophotometer that can accurately measure the absorbance at 492 nm
      Pipetting devices for accurate delivery of volumes required for the assay
      Cuvettes with optical properties suitable for use at 492 nm
      A constant-temperature water bath set at 37°C
      Microfuge for generating approximately 12,000 x g
      References
      ReferencesTwining, S.S. 1984. Anal. Biochem. 143, 30.
      Wiesner, R., et al. 1982. Anal. Biochem. 121, 290.
      Product Information
      Detection methodColorimetric
      Form200 Tests
      FormatCuvette or 96-well plate
      Kit containsA Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
      Quality LevelMQ100
      Applications
      Biological Information
      Assay time3 h
      Sample TypeBiological samples
      Physicochemical Information
      Sensitivity10 ng/ml
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Storage Conditions FTC-casein should be stored at -20°C and is stable for one year from the date of purchase. FTC-casein stored at 4°C is stable for 2-3 days. Several freeze-thaw cycles will lead to a minor increase in the background fluorescence, so following initial thawing, we suggest that this reagent be divided into 5 aliquots. FTC-casein is light sensitive and should be protected from light.
      Protease positive control should be stored at -20°C. As with many proteases, trypsin is susceptible to autolysis and you may notice a reduction in trypsin activity with time, even if stored at -20°C.
      Incubation buffer and assay buffer may be stored at 4°C or -20°C.
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Kit containsA Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      539125-1KIT 04055977195347

      Documentation

      Protease Assay Kit MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Protease Assay Kit Certificati d'Analisi

      TitoloNumero di lotto
      539125

      Riferimenti bibliografici

      Panoramica delle referenze
      Twining, S.S. 1984. Anal. Biochem. 143, 30.
      Wiesner, R., et al. 1982. Anal. Biochem. 121, 290.

      Brochure

      Titolo
      Kit SourceBook - 2nd Edition EURO

      Protocolli per l'uso

      Titolo
      User Protocol