Millipore Sigma Vibrant Logo

507861 PANSORBIN® Cells, Standardized

PANSORBIN® Cells, Standardized: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
View Products on Sigmaaldrich.com
507861
Visualizza prezzi e disponibilità

Panoramica

Replacement Information

Prezzi e disponibilità

Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
507861-25ML
Verifica della disponibilità in corso...
—
Disponibilità limitata Disponibilità limitata
A magazzino 
Fuori produzione
Disponibili quantità limitate
La disponibilità deve essere confermata
    Prodotti rimanenti: riceverà un nostro avviso
      Prodotti rimanenti: riceverà un nostro avviso
      Will advise
      Contatti il Servizio Clienti
      Contact Customer Service

      		 Fiala di plastica 25 ml
      Ricerca del prezzo in corso...
      Non è stato possibile trovare il prezzo
      La quantità minima deve essere un multiplo di
      Maximum Quantity is
      Al termine dell'ordine Maggiori informazioni
      Lei ha salvato ()
       
      —
      Richiedi il prezzo
      		 —
      507861-50ML
      Verifica della disponibilità in corso...
      —
      Disponibilità limitata Disponibilità limitata
      A magazzino 
      Fuori produzione
      Disponibili quantità limitate
      La disponibilità deve essere confermata
        Prodotti rimanenti: riceverà un nostro avviso
          Prodotti rimanenti: riceverà un nostro avviso
          Will advise
          Contatti il Servizio Clienti
          Contact Customer Service

          		 Fiala di plastica 50 ml
          Ricerca del prezzo in corso...
          Non è stato possibile trovare il prezzo
          La quantità minima deve essere un multiplo di
          Maximum Quantity is
          Al termine dell'ordine Maggiori informazioni
          Lei ha salvato ()
           
          —
          Richiedi il prezzo
          		 —
          Description
          OverviewPANSORBIN® Cells are heat-killed, formalin fixed Staphylococcus aureus cells that have a coat of protein A and have been pickled by the method of Kessler. Useful for mitogenic stimulation of B lymphocytes and for immunoprecipitation.
          Catalogue Number507861
          Brand Family Calbiochem®
          References
          ReferencesKierszenbaum, F., et al. 1991. Immunology 74, 317.
          Meikle, P.J., et al. 1991. J. Biol. Chem. 266, 22569.
          Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
          Murakami, H., et al. 1988. Biochem. J. 256, 917.
          Kessler, S.W. 1975. J. Immunol. 115, 1617.
          Product Information
          ActivityBinding capacity: ≥2 mg/ml
          FormLiquid
          FormulationSupplied as a ≥10% (w/v) Staphylococcus aureus cell suspension in PBS, 0.1% sodium azide, pH 7.2.
          Preservative0.1% sodium azide
          Quality LevelMQ100
          Applications
          Key Applications Immunoprecipitation
          Application NotesMost common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Numero di catalogo GTIN
          507861-25ML 04055977271959
          507861-50ML 04055977271966

          Documentation

          PANSORBIN® Cells, Standardized MSDS

          Titolo

          Scheda di sicurezza (MSDS) 

          PANSORBIN® Cells, Standardized Certificati d'Analisi

          TitoloNumero di lotto
          507861

          Riferimenti bibliografici

          Panoramica delle referenze
          Kierszenbaum, F., et al. 1991. Immunology 74, 317.
          Meikle, P.J., et al. 1991. J. Biol. Chem. 266, 22569.
          Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
          Murakami, H., et al. 1988. Biochem. J. 256, 917.
          Kessler, S.W. 1975. J. Immunol. 115, 1617.

          Citazioni

          Titolo
        • Kira Steigerwald, et al. (2005) The APC tumor suppressor promotes transcription-independent apoptosis in vitro. Molecular Cancer Research 3, 78-89.
          		•
          Scheda tecnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision14-February-2018 JSW
          ApplicationMost common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.
          DescriptionHeat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high-affinity interaction of protein A with the Fc domain of IgG. PANSORBIN® cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3).
          FormLiquid
          FormulationSupplied as a ≥10% (w/v) Staphylococcus aureus cell suspension in PBS, 0.1% sodium azide, pH 7.2.
          Recommended reaction conditions
          The procedures used for performing immunoprecipitations using PANSORBIN® cells are somewhat variable, but this protocol can serve as a general guideline. PANSORBIN® cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3). Prewashing: 1. Transfer desired amount of PANSORBIN® cells to a microfuge tube (typically 10-30 µl of a 10% PANSORBIN® cell suspension per immunoprecipitation). 2. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 3. Aspirate the supernatant. 4. Resuspend to the original volume or in 1.0 ml of cold lysis buffer, whichever is greater. Mix gently. 5. Repeat wash (steps 2-4). 6. Resuspend the final pellet to the original volume (from step 1) in lysis buffer. 7. If kept sterile, washed PANSORBIN® cells are stable at 4°C. Pre-Clearing (Optional): Pre-clearing with PANSORBIN® cells prior to immunoprecipitation removes material that binds nonspecifically, reducing the background. 1. Add 10-30 µl of washed PANSORBIN® cells to the lysate prior to incubation with the primary antibody. 2. Incubate for 1 h on ice, mixing occasionally. 3. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 4. Transfer the supernatant to another microfuge tube and discard the pellet. Alternatively, the lysate can be pre-cleared with PANSORBIN® cells which have been pre-bound to nonspecific antiserum (see Secondary Antibody pre-binding procedure). Primary Antibody: 1. Add the required amount of primary antibody to the lysate. 2. Incubate for at least 1 h on ice, mixing occasionally. 3. If a secondary antibody step is not required or desired, proceed to the PANSORBIN® Step described below. Secondary Antibody: In some cases, a secondary antibody will be necessary. For example, if the primary antibody is mouse IgG1, it is best to use a rabbit anti-mouse IgG as a secondary antibody. If desired, the secondary antibody can be pre-bound to the PANSORBIN® cells to eliminate the loss of antibody-antigen complexes which have not bound to the protein A. 1. Incubate the desired amount of secondary antibody with prewashed PANSORBIN® cells for 45-60 min on ice. 2. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 3. Aspirate the supernatant and wash the lysis buffer. 4. Resuspend in lysis buffer to a volume equal to the original volume of PANSORBIN® cells which were added. Alternatively, the unbound secondary antibody and PANSORBIN® cells can be incubated with the sample sequentially, following primary antibody incubation. PANSORBIN® Step: 1. Add at least 10X the amount of PANSORBIN® cells (alone or bound to secondary antibody) needed to precipitate the primary (or secondary) antibody. PANSORBIN® cells bind about 2 mg of human IgG/ml. Typically, add 10-30 µl of PANSORBIN® cells per immunoprecipitation. This quantity gives a visible pellet. 2. Incubate for 1-2 h on ice, mixing regularly. 3. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 4. Wash PANSORBIN® pellet 2-3 times with lysis buffer. 5. If samples are to be loaded onto a polyacrylamide gel, the PANSORBIN® pellet can be resuspended in sample buffer, boiled for 10 min, centrifuged quickly (30 s in a microfuge at 20°C). Load the supernatant onto the gel. If a high background is observed, it may be necessary to pre-clear lysates, prebind the antibody to PANSORBIN® cells, or use a stronger lysis buffer (e.g. RIPA). This protocol is meant to serve as a guideline and may need to be modified for specific applications.
          ActivityBinding capacity: ≥2 mg/ml
          Preservative0.1% sodium azide
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesKierszenbaum, F., et al. 1991. Immunology 74, 317.
          Meikle, P.J., et al. 1991. J. Biol. Chem. 266, 22569.
          Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
          Murakami, H., et al. 1988. Biochem. J. 256, 917.
          Kessler, S.W. 1975. J. Immunol. 115, 1617.
          Citation
        • Kira Steigerwald, et al. (2005) The APC tumor suppressor promotes transcription-independent apoptosis in vitro. Molecular Cancer Research 3, 78-89.
          		•

          Prodotti e applicazioni correlate

          Alternative Products

          Numero di catalogo Descrizione  
          507858 PANSORBIN® Cells Prezzi e disponibilità
          507862 PANSORBIN® Cells, Lyophilized Prezzi e disponibilità

          Related Products

          Numero di catalogo Descrizione  
          507858 PANSORBIN® Cells Prezzi e disponibilità

          Prodotti correlati per: Brand Facete

          PANSORBIN®

          Categorie

          Life Science Research > Protein Detection and Quantification > Immunodetection Support Products > Protein A & Protein G Reagents