71091 Sigma-AldrichNovaTaq™ Hot Start DNA Polymerase
NovaTaq™ Hot Start DNA Polymerase is a heat-activatable modified form of recombinant Taq DNA polymerase
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Prezzi e disponibilità
Numero di catalogo | Disponibilità | Confezionamento | Qtà/conf | Prezzo | Quantità | |
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71091-3 |
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Bottiglia di vetro | 250 u |
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71091-4 |
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Bottiglia di vetro | 1250 u |
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Source | Recombinant Thermus aquaticus DNA polymerase expressed in E. coli |
Concentration | 5.0 U/µl |
Endonuclease | None detected |
Exonuclease | None detected |
Amplification effiency | Functional PCR |
Storage | –20°C |
Features and benefits
- Higher PCR specificity and yield
- Improved low-copy target amplification
- Ambient temperature setup compatible with automation
- Target amplification of up to 5 kbp
- Ideal for quantitative and high-throughput PCR applications
Product Information | ||
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Unit of Definition | One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 72°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-³²P]dCTP, and 12.5 µg activated salmon sperm DNA in a volume of 50 µl. | |
• | 250 U or 5 × 250 U | NovaTaq Hot Start DNA Polymerase (5 U/µl) |
• | 1.5 ml or 5 × 1.5 ml | 10X NovaTaq Hot Start Buffer |
• | 1.5 ml or 5 × 1.5 ml | 25 mM MgCl₂ |
Declaration | Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA. | |
Quality Level | MQ100 |
Applications | |
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Application | NovaTaq™ Hot Start DNA Polymerase is a heat-activatable modified form of recombinant Taq DNA polymerase |
Storage and Shipping Information | |
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Ship Code | Dry Ice Only |
Toxicity | Multiple Toxicity Values, refer to MSDS |
Storage | -20°C |
Do not freeze | Ok to freeze |
Global Trade Item Number | |
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Numero di catalogo | GTIN |
71091-3 | 04055977271751 |
71091-4 | 04055977271768 |