Millipore Sigma Vibrant Logo

71842 KOD Hot Start Master Mix

Ready-to-use 2X PCR Master Mix for high fidelity PCR

KOD Hot Start Master Mix: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
View Products on Sigmaaldrich.com
71842
Visualizza prezzi e disponibilità

Panoramica

Replacement Information

Prezzi e disponibilità

Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
71842-3
Verifica della disponibilità in corso...
—
Disponibilità limitata Disponibilità limitata
A magazzino 
Fuori produzione
Disponibili quantità limitate
La disponibilità deve essere confermata
    Prodotti rimanenti: riceverà un nostro avviso
      Prodotti rimanenti: riceverà un nostro avviso
      Will advise
      Contatti il Servizio Clienti
      Contact Customer Service

      		 Fiala di plastica 100 rxn
      Ricerca del prezzo in corso...
      Non è stato possibile trovare il prezzo
      La quantità minima deve essere un multiplo di
      Maximum Quantity is
      Al termine dell'ordine Maggiori informazioni
      Lei ha salvato ()
       
      —
      Richiedi il prezzo
      		 —
      71842-4
      Verifica della disponibilità in corso...
      —
      Disponibilità limitata Disponibilità limitata
      A magazzino 
      Fuori produzione
      Disponibili quantità limitate
      La disponibilità deve essere confermata
        Prodotti rimanenti: riceverà un nostro avviso
          Prodotti rimanenti: riceverà un nostro avviso
          Will advise
          Contatti il Servizio Clienti
          Contact Customer Service

          		 Bottiglia di vetro 500 rxn
          Ricerca del prezzo in corso...
          Non è stato possibile trovare il prezzo
          La quantità minima deve essere un multiplo di
          Maximum Quantity is
          Al termine dell'ordine Maggiori informazioni
          Lei ha salvato ()
           
          —
          Richiedi il prezzo
          		 —
          Description
          OverviewPCR involves replication of a DNA template by a thermostable DNA polymerase. The processivity, specificity, and fidelity of the polymerase enzyme used can influence the efficiency, reproducibility, and yield of the PCR reaction. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. Fidelity is critical when accurate sequence amplification of the gene target is needed, for example, when direct sequencing or cloning for downstream protein expression. Unwarranted mutation could severely impact your studies. Our analysis has shown that KOD enzymes are an easy choice for fast, accurate and high-yielding PCR. EMD Millipore's molecular biologists work to develop and formulate polymerases offering the highest specificity, fidelity and yield during PCR amplification. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility.
          KOD Hot Start Master Mix* is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4. The Master Mix simplifies PCR set-up, offering time savings, consistency, and minimal risk of contamination. The mix is ideal for use in high-throughput applications. Simply add KOD Hot Start Master Mix to an equal volume of sample containing DNA template and primers. The final diluted reaction contains 1 U KOD Hot Start DNA Polymerase per 50 µl reaction. The smaller available size provides sufficient master mix for 100 (50 µl scale), or 250 (20 µl scale) reactions, while the larger size is adequate for 500 (50 µl scale) or 1250 (20 µl scale) reactions.

          Features and Benefits:

          • Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
          • Amplifies genomic DNA templates up to 12 kbp
          • Amplifies plasmid and lambda DNA template up to 21 kbp
          • Eliminates mispriming and primer-dimer formation
          • Conveient ambient-temperature setup compatible with automation
          • KOD Hot Start Buffer ensures optimal PCR performance over a wide range of targets.

            *Manufactured by Toyobo and distributed by EMD. Not available from Merck in Japan.

            Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
          Catalogue Number71842
          Brand Family Novagen®
          References
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [<Sup>3</Sup>H]dTTP), and 150 µg/ml activated calf thymus DNA.

          •

          		2 × 1.25 ml or 10 × 1.25 mlKOD Hot Start Master Mix

           

          References:

          Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem. 66 (10), 2194. Fujii, S., et al. 1999. J. Mol. Biol. 289, 835.

          Declaration* Manufactured by Toyobo and distributed by EMD. Not available from Merck in Japan. Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Numero di catalogo GTIN
          71842-3 07790788053475
          71842-4 07790788053482