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324725 Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli

Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and hybrid. Suitable for deglycosylation of native proteins.

MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.

Sinonimi: Endo-β-N-acetylglucosaminidase F1, Endo F1

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324725
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Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
324725-700MIU
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      Description
      OverviewRecombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.
      Note: 1 mU = 1 milliunit.
      Catalogue Number324725
      Brand Family Calbiochem®
      SynonymsEndo-β-N-acetylglucosaminidase F1, Endo F1
      References
      ReferencesTarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
      Product Information
      Activity≥17 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5.
      EC number3.2.1.96
      FormLiquid
      FormulationIn 20 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥16 units/mg protein
      Physicochemical Information
      ContaminantsProteases: none detected.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      324725-700MIU 04055977215991

      Documentation

      Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli Certificati d'Analisi

      TitoloNumero di lotto
      324725

      Riferimenti bibliografici

      Panoramica delle referenze
      Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

      Citazioni

      Titolo
    • Emily M. Kwan, et al. (2005) N-Glycosidase-carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation. Protein Engineering Design and Selection 18, 497-501.
      		•
      Scheda tecnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-September-2007 RFH
      SynonymsEndo-β-N-acetylglucosaminidase F1, Endo F1
      DescriptionRecombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185), and therefore is more suitable for deglycosylation of native proteins.
      FormLiquid
      FormulationIn 20 mM Tris-HCl, pH 7.5.
      Recommended reaction conditions50 mM sodium phosphate buffer, pH 5.5
      EC number3.2.1.96
      ContaminantsProteases: none detected.
      Specific activity≥16 units/mg protein
      Activity≥17 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesTarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
      Citation
    • Emily M. Kwan, et al. (2005) N-Glycosidase-carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation. Protein Engineering Design and Selection 18, 497-501.
      		•