Millipore Sigma Vibrant Logo

324682 Elastase, Porcine Pancreas, High Purity, Crystallized

Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.

MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
View Products on Sigmaaldrich.com
324682
Visualizza prezzi e disponibilità

Panoramica

Replacement Information

Prezzi e disponibilità

Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
324682-1000U
Verifica della disponibilità in corso...
—
Disponibilità limitata Disponibilità limitata
A magazzino 
Fuori produzione
Disponibili quantità limitate
La disponibilità deve essere confermata
    Prodotti rimanenti: riceverà un nostro avviso
      Prodotti rimanenti: riceverà un nostro avviso
      Will advise
      Contatti il Servizio Clienti
      Contact Customer Service

      		 Fiala di plastica 1000 u
      Ricerca del prezzo in corso...
      Non è stato possibile trovare il prezzo
      La quantità minima deve essere un multiplo di
      Maximum Quantity is
      Al termine dell'ordine Maggiori informazioni
      Lei ha salvato ()
       
      —
      Richiedi il prezzo
      		 —
      324682-250U
      Verifica della disponibilità in corso...
      —
      Disponibilità limitata Disponibilità limitata
      A magazzino 
      Fuori produzione
      Disponibili quantità limitate
      La disponibilità deve essere confermata
        Prodotti rimanenti: riceverà un nostro avviso
          Prodotti rimanenti: riceverà un nostro avviso
          Will advise
          Contatti il Servizio Clienti
          Contact Customer Service

          		 Fiala di plastica 250 u
          Ricerca del prezzo in corso...
          Non è stato possibile trovare il prezzo
          La quantità minima deve essere un multiplo di
          Maximum Quantity is
          Al termine dell'ordine Maggiori informazioni
          Lei ha salvato ()
           
          —
          Richiedi il prezzo
          		 —
          Description
          OverviewNative elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.
          Catalogue Number324682
          Brand Family Calbiochem®
          References
          ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
          Mandl, I. 1962. Methods in Enzymol. 5, 665.
          Product Information
          CAS number39445-21-1
          Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=324699">Cat. No. 324699</a>) per min at 25°C, pH 8.0.
          EC number3.4.21.36
          FormLyophilized
          FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
          Quality LevelMQ100
          Applications
          Biological Information
          Specific Activity≥25 units/mg protein
          Physicochemical Information
          ContaminantsChymotrypsin: ≤0.2%
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSTS5052000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Numero di catalogo GTIN
          324682-1000U 04055977196832
          324682-250U 07790788054380

          Documentation

          Elastase, Porcine Pancreas, High Purity, Crystallized MSDS

          Titolo

          Scheda di sicurezza (MSDS) 

          Elastase, Porcine Pancreas, High Purity, Crystallized Certificati d'Analisi

          TitoloNumero di lotto
          324682

          Riferimenti bibliografici

          Panoramica delle referenze
          Largman, C. et al. 1976. Biochemestry 15, 2491.
          Mandl, I. 1962. Methods in Enzymol. 5, 665.
          Scheda tecnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-July-2010 JSW
          DescriptionNative elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.
          FormLyophilized
          FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
          CAS number39445-21-1
          RTECSTS5052000
          EC number3.4.21.36
          ContaminantsChymotrypsin: ≤0.2%
          Specific activity≥25 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-pNA (Cat. No. 324699) per min at 25°C, pH 8.0.
          SolubilitydH₂O (10 mg/ml)
          Storage Protect from moisture
          -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
          Toxicity Standard Handling
          Merck USA index14, 3535
          ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
          Mandl, I. 1962. Methods in Enzymol. 5, 665.

          Prodotti e applicazioni correlate

          Related Products

          Numero di catalogo Descrizione  
          164598 Aminopeptidase M, Porcine Kidney Prezzi e disponibilità
          324708 Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi Prezzi e disponibilità
          324713 Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus Prezzi e disponibilità
          324715 Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes Prezzi e disponibilità
          516360 Pepsin, Porcine Stomach Mucosa Prezzi e disponibilità
          650211 Trypsin, Excision Grade, Bovine Pancreas Prezzi e disponibilità

          Categorie

          Life Science Research > Proteins and Enzymes > Other Enzymes