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S7865 CpGenome™ Rat Methylated and Unmethylated Genomic DNA Standard Set

Useful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.

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S7865
5 µg per vial  
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      Replacement Information
      Description
      Catalogue NumberS7865
      Brand Family Upstate
      Trade Name
      • CpGenome
      • Upstate
      DescriptionCpGenome™ Rat Methylated and Unmethylated Genomic DNA Standard Set
      OverviewThe CpGenome™ Rat Methylated and Unmethylated Genomic DNA Standard Set contains one vial of unmethylated DNA and one vial of methylated DNA generated from the same genomic DNA source.

      The CpGenome™ Rat Unmethylated Genomic DNA Standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase. This process results in a DNA standard which contains ≥ 95% unmethylated cytosines across the genome in both heterochromatic and euchromatic regions.

      The CpGenome™ Rat Methylated Genomic DNA Standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase followed by a treatment with a CpG Methylase. This process results in a DNA standard which contains ≥ 95% methylated cytosines across the genome in both heterochromatic and euchromatic regions.
      References
      Product Information
      HS Code2934 99 99
      Presentation5 µg DNA in buffer containing 10mM Tris-HCl pH 8.0, 0.1mM EDTA
      Quality LevelMQ100
      Applications
      ApplicationUseful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.
      Biological Information
      Species Reactivity
      • Rat
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceQuantitative Methylation Specific PCR (qMSP):
      Bisulfite converted unmethylated and methylated rat genomic DNA are assessed for methylation status at various loci within the genome.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt aliqout and store at -20°C to avoid multiple freeze thaws which can fragment DNA. For best quality, limit freeze and thaw of the same –20°C DNA aliquot to no more than 3 times. For longer term storage, the DNA can be stored at -80°C. With proper storage and protection from excess freeze-thaw cycles, this product is stable for up to two years from the date of receipt.
      Packaging Information
      Material Size5 µg per vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      S7865 04055977118216