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17-656 ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set

This ChIPAb+ Sox-2, clone 6F1.2 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Key Applications: Western Blotting, Dot Blot, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP) Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology, Transcription Factors
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17-656
25 assays  25 assays per set. Recommended use: ~4 μg antibody per chromatin immunoprecipitation (dependent upon biological context).
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      Tabella delle specifiche principali

      Species ReactivityKey Applications
      H, MWB, DB, ICC, ChIP
      Description
      Catalogue Number17-656
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set
      Alternate Names
      • Transcription factor SOX-2
      • SOX2
      Background InformationAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+ Sox-2, clone 6F1.2 antibody/primer set includes the Sox-2, clone 6F1.2 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 95 bp region within the promoter of the human nanog gene. The Sox-2, clone 6F1.2 antibody and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Sox-2-associated chromatin.

      Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain pluripotent potential. It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS.
      References
      Product Information
      Components
      • Anti-Sox-2, clone 6F1.2 (mouse monoclonal IgG), Part No. CS204373.
        One vial containing 100 μg of protein A purified antibody in 140 μL of 0.02 M phosphate buffer pH 7.6 with 0.25 M NaCl containing 0.1% sodium azide, and 30% glycerol. Store at -20°C.
      • Normal Mouse IgG, Part No. CS200621.
        One vial containing 125 μg of purified mouse IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
      • ChIP Primers, nanog promoter,
        Part No. CS204374. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human nanog.
        Store at -20°C.
        FOR: GTT CTG TTG CTC GGT TTT CT
        REV: TCC CGT CTA CCA GTC TCA CC
      FormatPurified
      Control
      • Includes negative control mouse IgG antibody and primers specific for human nanog promoter region.
      Quality LevelMQ100
      Applications
      ApplicationThis ChIPAb+ Sox-2, clone 6F1.2 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • Western Blotting
      • Dot Blot
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesPlease refer to the EZ-Magna G ChIP™ (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details.
      Biological Information
      ImmunogenRecombinant GST Fusion protein.
      Clone6F1.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Sox-2, MW: ~34 kDa.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, mouse.
      Not tested in other species.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
      Gene Symbol
      • SOX2
      • ANOP3
      • MGC2413
      • MCOPS3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P48431 # Can form ternary complexes with OCT-1 or OCT-3 but acts as a transcriptional activator of FGF-4 enhancer DNA sequences only in conjunction with OCT-3 (By similarity).

      SIZE: 317 amino acids; 34310 Da

      SUBCELLULAR LOCATION: Nucleus.

      DISEASE: SwissProt: P48431 # Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. MCOPS3 is characterized by the association of microphthalmia or clinical anophthalmia with anomalies such as microcephaly, short stature, hypogonadotropic hypogonadism, esophageal atresia and neurologic manifestations.

      SIMILARITY: SwissProt: P48431 ## Contains 1 HMG box DNA-binding domain.
      Molecular Weight~34 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Sonicated chromatin prepared from Ntera 2 cells (3 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either a normal mouse IgG, or Anti-Sox-2, clone 6F1.2 antibody and the Magna ChIP™ G Kit (Cat. No. 17-611). Successful immunoprecipitation of Sox-2-associated DNA fragments was verified by qPCR using ChIP Primers, nanog promoter (Figure 1).
      Please refer to the EZ-Magna ChIP™ G (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 assays
      Material Package25 assays per set. Recommended use: ~4 μg antibody per chromatin immunoprecipitation (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      17-656 04053252284472

      Documentation

      ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set Certificati d'Analisi

      TitoloNumero di lotto
      ChIPAb+ Sox-2, clone 6F1.2 - 2142699 2142699
      ChIPAb+ Sox-2, clone 6F1.2 - 2450698 2450698
      ChIPAb+ Sox-2, clone 6F1.2 - 1947173 1947173
      ChIPAb+ Sox-2, clone 6F1.2 - 2067372 2067372
      ChIPAb+ Sox-2, clone 6F1.2 - 2205936 2205936
      ChIPAb+ Sox-2, clone 6F1.2 - 3197837 3197837
      ChIPAb+ Sox-2, clone 6F1.2 - 3225602 3225602
      ChIPAb+ Sox-2, clone 6F1.2 - 3437917 3437917
      ChIPAb+ Sox-2, clone 6F1.2 - 3862821 3862821
      ChIPAb+ Sox-2, clone 6F1.2 - 4163819 4163819
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      Riferimenti bibliografici

      Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
      SOX2 is a cancer-specific regulator of tumour initiating potential in cutaneous squamous cell carcinoma.
      Siegle, JM; Basin, A; Sastre-Perona, A; Yonekubo, Y; Brown, J; Sennett, R; Rendl, M; Tsirigos, A; Carucci, JA; Schober, M
      Nature communications  5  4511  2014

      Mostra il sommario       		25077433 25077433

      Brochure

      Titolo
      Advance your Epigenetics Research
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

      Scheda tecnica

      Titolo
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

      Prodotti e applicazioni correlate

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      Categorie

      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies
      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies