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218783 Caspase-1, Human, Recombinant, E. coli

Caspase-1, Human, Recombinant, is expressed in E. coli and is fused at the N-terminus to a His•Tag® sequence.

MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.

Sinonimi: IL-1β Converting Enzyme, ICE

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218783
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Replacement Information

Prezzi e disponibilità

Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
218783-3000U
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      Description
      OverviewRecombinant, human caspase-1 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.
      Catalogue Number218783
      Brand Family Calbiochem®
      SynonymsIL-1β Converting Enzyme, ICE
      References
      ReferencesThornberry, N.A. 1994. Methods Enzymol. 244, 615.
      Thornberry, N.A., et al. 1992. Nature 356, 768.
      Product Information
      Activity≥50,000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=400025">Cat. No. 400025</a>) per min at 30°C, pH 7.4.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥25,000 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      218783-3000U 04055977218756

      Documentation

      Caspase-1, Human, Recombinant, E. coli MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Caspase-1, Human, Recombinant, E. coli Certificati d'Analisi

      TitoloNumero di lotto
      218783

      Riferimenti bibliografici

      Panoramica delle referenze
      Thornberry, N.A. 1994. Methods Enzymol. 244, 615.
      Thornberry, N.A., et al. 1992. Nature 356, 768.

      Brochure

      Titolo
      Caspases and other Apoptosis Related Tools Brochure

      Citazioni

      Titolo
    • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
      		•
      Scheda tecnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-March-2009 JSW
      SynonymsIL-1β Converting Enzyme, ICE
      DescriptionRecombinant, human caspase-1 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
      Purity≥90% by SDS-PAGE
      Specific activity≥25,000 units/mg protein
      Activity≥50,000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-pNA (Cat. No. 400025) per min at 30°C, pH 7.4.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesThornberry, N.A. 1994. Methods Enzymol. 244, 615.
      Thornberry, N.A., et al. 1992. Nature 356, 768.
      Citation
    • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
      		•