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SCT072 BioTracker NIR-ASM NQO1 Live Cell Dye

Live cell imaging fluorescent probe for near-infrared imaging of NQO1 enzyme in cancer cells in vitro and in vivo

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SCT072
1 mg  
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      Replacement Information
      Description
      Catalogue NumberSCT072
      DescriptionBioTracker NIR-ASM NQO1 Live Cell Dye
      OverviewNAD(P)H quinone oxidoreductase 1 (NQO1) is a cancer-specific target enzyme and accurate detection of NQO1 activity with high sensitivity and selectivity is useful for the early diagnosis of cancer. NQO1 is a two-electron reductase responsible for the detoxification of xenobiotics such as quinones and is abnormally overexpressed in many tumors and intimately linked with multiple carcinogenic processes. compared to healthy tissue, NQO1 expressed at levels of 5−200-fold above in tumors, such as the breast12, lung13,14, prostate15, stomach16, colon17, pancreas18, head, and neck cancer19. Consistent with this, NQO1 has been extensively explored to achieve highly selective and sensitive detection or visualization of tumor-associated events in different cancers.

      BioTracker NIR-ASM NQO1 Live Cell Dye demonstrated high selectivity for NQO1 over the other enzymes/substrates such as glutathione, aldehyde dehydrogenase 1, gamma-glutamyl transferase, glutathione peroxidase, apurinic/apyrimidinic endonuclease, cystathionine-β-synthase, cathepsin L, glutathione S-transferase, NADH alone and the combination of nitroreductase and NQO1. Fluorescence only resulted from the specific cleavage of the probe by NQO1 in the presence of its co-factor NADH. NIR-ASM dye was not toxic to both normal and cancer cells even at higher concentrations (up to 100 μM). A high-intensity distribution of signals was obtained for NIR-ASM dye activation in each of the two NQO1-positive cancer cell lines (A549 and H460), while the negative normal cell lines IMR90 and HUVECs produced minimal fluorescence.

      Spectral properties:
      Excitation: 488 nm
      Emission: 644 nm

      References:
      Punganuru S.R. et al. Characterization of a highly specific NQO1-activated nearinfrared fluorescent probe and its application for in vivo tumor imaging. Sci. Reports. 2019, 9:8577.
      Alternate Names
      • Live cell imaging probe
      • NQO1 probe
      References
      Product Information
      Detection methodFluorescence
      PresentationLyophilized
      Applications
      ApplicationLive cell imaging fluorescent probe for near-infrared imaging of NQO1 enzyme in cancer cells in vitro and in vivo
      Key Applications
      • Cell Based Assays
      Application NotesLive cell fluorescent imaging
      Biological Information
      Molecular Weight521.6
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssurancePurity: ≥ 94% confirmed by HPLC. Structure confirmed by HNMR, LC-MS and elemental analysis
      Molar Mass: 521.6 g/mol
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker NIR-ASM NQO1 Live Cell Dye at -20°C, desiccate and protect from light
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      SCT072 04061841441045