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OP07 Anti-v-Src (Ab-1) Mouse mAb (327)

This Anti-v-Src (Ab-1) Mouse mAb (327) is validated for use in Immunoblotting, Immunoprecipitation, Frozen Sections, Immunofluorescence, Paraffin Sections for the detection of v-Src (Ab-1).

Anti-v-Src (Ab-1) Mouse mAb (327): MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
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OP07
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Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Prezzi e disponibilità

Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
OP07-100UG
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      Description
      OverviewRecognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.
      Catalogue NumberOP07
      Brand Family Calbiochem®
      References
      ReferencesCourtneidge, S.A. and Heber, A. 1987. Cell 50, 1031.
      Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
      Courtneidge, S.A. 1985. EMBO J. 4, 1471.
      Whitman, M., et al. 1985. Nature 315, 239
      Bolen, J.B., et al. 1984. Cell 38, 767.
      Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
      Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
      Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
      Collett, M.S., et al. 1978. Cell 15, 1363.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
      Positive controlNIH/3T3 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmune Kinase Reaction Bolen, J.B. et al. 1985. Proc. Natl. Acad. Sci. 82, 7275. Immunoblotting Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Grandori, C., and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Immunoprecipitation Grandori, C., and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Original Clone Lipsich, L.A., et al. 1983. J. Virol. 48, 352.
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Not Frozen Sections
      Not Immunofluorescence
      Not Paraffin Sections
      Application NotesImmunoblotting (2.5 µg/ml, see application references)
      Immunoprecipitation (1 µg antibody or use Cat. No. OP07A; see application references)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Paraffin Sections (not recommended)
      Application Commentspp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active src kinase (see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified v-Src
      Epitopewithin the SH3 domain of v-Src and c-Src
      Clone327
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      OP07-100UG 04055977225006

      Documentation

      Anti-v-Src (Ab-1) Mouse mAb (327) MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Anti-v-Src (Ab-1) Mouse mAb (327) Certificati d'Analisi

      TitoloNumero di lotto
      OP07

      Riferimenti bibliografici

      Panoramica delle referenze
      Courtneidge, S.A. and Heber, A. 1987. Cell 50, 1031.
      Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
      Courtneidge, S.A. 1985. EMBO J. 4, 1471.
      Whitman, M., et al. 1985. Nature 315, 239
      Bolen, J.B., et al. 1984. Cell 38, 767.
      Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
      Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
      Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
      Collett, M.S., et al. 1978. Cell 15, 1363.

      Citazioni

      Titolo
    • Meenalakshmi M. Mariappan, et al. (2005) Phospholipase Cγ-Erk Axis in Vascular Endothelial Growth Factor-Induced Eukaryotic Initiation Factor 4E Phosphorylation and Protein Synthesis in Renal Epithelial Cells. Journal of Biological Chemistry 280, 28402-28411.
    • Justin M. Summy, et al. (2005) AP23846, a novel and highly potent Src family kinase inhibitor, reduces vascular endothelial growth factor and interleukin-8 expression in human solid tumor cell lines and abrogates downstream angiogenic processes. Molecular Cancer Therapeutics 4, 1900-1911.
    • Jose G. Trevino, et al. (2005) Expression and activity of Src regulate interleukin-8 expression in pancreatic adenocarcinoma cells: implications for angiogenesis. Cancer Research 65, 7214-7222.
      		•
      Scheda tecnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 RFH
      ApplicationImmunoblotting (2.5 µg/ml, see application references)
      Immunoprecipitation (1 µg antibody or use Cat. No. OP07A; see application references)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein.
      BackgroundThe major translational product of the avian sarcoma virus has been designated pp60src. Avian sarcoma virus pp60src possesses a characteristic protein kinase activity with specificity for tyrosine residues. The mammalian cellular homologue of pp60src, designated pp60c-src, exhibits a similar protein tyrosine kinase activity which can be activated by interaction with polyoma virus-encoded middle T antigen. Immunoprecipitates of middle T antigen also contain phosphatidylinositol kinase activity coinciding with the presence of a 81 kDa (p81) protein. The p81 is phosphorylated by activated pp60c-src and is thought to be the phosphatidylinositol kinase.
      HostMouse
      Immunogenpurified v-Src
      Epitopewithin the SH3 domain of v-Src and c-Src
      Clone327
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlNIH/3T3 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      Commentspp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active src kinase (see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesCourtneidge, S.A. and Heber, A. 1987. Cell 50, 1031.
      Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
      Courtneidge, S.A. 1985. EMBO J. 4, 1471.
      Whitman, M., et al. 1985. Nature 315, 239
      Bolen, J.B., et al. 1984. Cell 38, 767.
      Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
      Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
      Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
      Collett, M.S., et al. 1978. Cell 15, 1363.
      Citation
    • Meenalakshmi M. Mariappan, et al. (2005) Phospholipase Cγ-Erk Axis in Vascular Endothelial Growth Factor-Induced Eukaryotic Initiation Factor 4E Phosphorylation and Protein Synthesis in Renal Epithelial Cells. Journal of Biological Chemistry 280, 28402-28411.
    • Justin M. Summy, et al. (2005) AP23846, a novel and highly potent Src family kinase inhibitor, reduces vascular endothelial growth factor and interleukin-8 expression in human solid tumor cell lines and abrogates downstream angiogenic processes. Molecular Cancer Therapeutics 4, 1900-1911.
    • Jose G. Trevino, et al. (2005) Expression and activity of Src regulate interleukin-8 expression in pancreatic adenocarcinoma cells: implications for angiogenesis. Cancer Research 65, 7214-7222.
      		•
      Application referencesImmune Kinase Reaction Bolen, J.B. et al. 1985. Proc. Natl. Acad. Sci. 82, 7275. Immunoblotting Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Grandori, C., and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Immunoprecipitation Grandori, C., and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Original Clone Lipsich, L.A., et al. 1983. J. Virol. 48, 352.