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07-952 Anti-trimethyl Histone H3 (Lys79) Antibody

Anti-trimethyl Histone H3 (Lys79) Antibody is a rabbit polyclonal antibody for detection of trimethyl Histone H3 (Lys79). It has been tested for use in Western blotting, Dot blots, and Multiplex assays.

Anti-trimethyl Histone H3 (Lys79) Antibody: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones, Chromatin Biology Gene Symbol: HIST3H3, H3FT, H3t, H3T, H3/g, H3.4, H3/t UniProt Number: Q16695
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07-952
100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, DB, MplexRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-952
      DescriptionAnti-trimethyl Histone H3 (Lys79) Antibody
      Alternate Names
      • H3 histone family, member T
      • histone 3, H3
      • histone cluster 3, H3
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Lysine methylation occurs in three distinct states, having either one (me1), two (me2) or three (me3) methyl groups attached to the amine group of the lysine side chain. Methylation of Lysine 79 in Histone 3 acts as a marker of inactive chromatin regions. Methylation by Dot1 methylase on lysine-79 (Lys79) of Histone H3 is criticial for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • HeLa acid extract lysate and recombinant Histone H3
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-trimethyl Histone H3 (Lys79) Antibody is a rabbit polyclonal antibody for detection of trimethyl Histone H3 (Lys79). It has been tested for use in Western blotting, Dot blots, and Multiplex assays.
      Key Applications
      • Western Blotting
      • Dot Blot
      • Multiplexing
      Application Notes
      Dot Blot Specificity Analysis:
      AbSurance™ Histone H3 Antibody Specificity Arrays (Cat. #: 16-667 and 16-665) containing peptides to all key histone modification sites, were probed with Anti-trimethyl Histone H3 (Lys79) Antibody Antibody ; dilution 1:5000. Proteins were visualized using a goat anti-mouse IgG (H+L) conjugated to HRP and a chemiluminescence detection system.


      Multiplex Immunoassay
      Specificity of interaction of anti-trimethyl histone H3 Lys79 antibody was assayed using the Luminex® multiplex immunoassay platform. Anti-trimethyl-Histone H3 (Lys79) diluted to 0.01 mg/ml concentration was incubated with a cocktail of xMAP microspheres conjugated to the following histone H3 peptides, monomethyl Lys79, unmodified Lys79, trimethyl Lys 27, trimethyl Lys 9, trimethyl Lys79 processed and detected using standard xMAP protocols.
      Biological Information
      ImmunogenLinear Peptide corresponding to human Histone H3 methylated at Lys79.
      EpitopeLys79
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Histone H3 methylated at Lys79.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with human. Broad species reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • H3t
      • H3T
      • H3/g
      • H3.4
      • H3/t
      Modifications
      • Methylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SIZE: 136 amino acids; 15508 Da

      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Expressed in testicular cells.

      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).

      SIMILARITY: Belongs to the histone H3 family.
      Molecular Weight~ 17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract lysate.

      Western Blot Analysis: 0.1 µg/ml of this antibody detected Histone H3 on 10 µg of HeLa acid extract lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      07-952 04053252370687

      Documentation

      Anti-trimethyl Histone H3 (Lys79) Antibody MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Anti-trimethyl Histone H3 (Lys79) Antibody Certificati d'Analisi

      TitoloNumero di lotto
      Anti-trimethyl Histone H3 (Lys79) - 2115686 2115686
      Anti-trimethyl Histone H3 (Lys79) - 2044808 2044808
      Anti-trimethyl Histone H3 (Lys79) - 2207265 2207265
      Anti-trimethyl Histone H3 (Lys79) - 2361815 2361815
      Anti-trimethyl Histone H3 (Lys79) - 3063865 3063865
      Anti-trimethyl Histone H3 (Lys79) - DAM1778280 DAM1778280
      Anti-trimethyl Histone H3 (Lys79) - DAM1831371 DAM1831371
      Anti-trimethyl Histone H3 (Lys79) - JBC1870043 JBC1870043
      Anti-trimethyl Histone H3 (Lys79) - NRG1697039 NRG1697039
      Anti-trimethyl Histone H3 (Lys79) -2594406 2594406

      Riferimenti bibliografici

      Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
      A comprehensive epigenome map of Plasmodium falciparum reveals unique mechanisms of transcriptional regulation and identifies H3K36me2 as a global mark of gene suppression.
      Karmodiya, K; Pradhan, SJ; Joshi, B; Jangid, R; Reddy, PC; Galande, S
      Epigenetics & chromatin  8  32  2015

      Mostra il sommario       		26388940 26388940

      Informazioni tecniche

      Titolo
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics