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Scelga pannelli su misura e kit premiscelati, OPPURE le microsfere MAPmates™ per la trasduzione del segnale
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La nostra ampia gamma comprende pannelli multiplex che consentono di scegliere, nell'ambito del pannello, gli analiti più adatti per le Sue esigenze. Oppure Lei può scegliere le citochine premiscelate o i kit single plex.
Kit e MAPmates™ per studi di trasduzione del segnale
Scelga i kit premiscelati che permettono di esplorare interi processi e pathway. Oppure progetti kit su misura scegliendo le microsfere MAPmates™ single plex e seguendo le linee guida fornite.
Le seguenti MAPmates™ non possono essere dosate insieme: -MAPmates™ che richiedono tamponi differenti per l'analisi. -MAPmate™ fosfo-specifiche e totali, es GSK3β totale e GSK3β (Ser 9). -MAPmates™ PanTyr e sito-specifiche come fosfo-recettore EGF e fosfo-STAT1 (Tyr701). -Più di 1 fosfo-MAPmate™ per un solo bersaglio (Akt, STAT3). -GAPDH e β-Tubulina non possono essere miscelati con kit o MAPmates™ contenenti panTyr.
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Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Opzione salva-spazio I Clienti che ordinano diversi kit possono scegliere di ridurre lo spazio necessario per lo stoccaggio, rinunciando al confezionamento del kit e ricevendo i vari reagenti per il saggio multiplex in buste di plastica.
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Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size, is a rabbit polyclonal antibody for detection of Histone H3 trimethylated at lysine 36 (H3K36me3). This purified Ab is specificity verified by dot blot (DB) and validated in WB, ICC. Small pack size is ideal for screening applications.
More>>Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size, is a rabbit polyclonal antibody for detection of Histone H3 trimethylated at lysine 36 (H3K36me3). This purified Ab is specificity verified by dot blot (DB) and validated in WB, ICC. Small pack size is ideal for screening applications. Less<<
MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Methylation occurs on histone H3 at lysine 4, 9, 27, 36, and 79 residues. Previous studies have reported that methylation of H3 lysine 9 provides a binding site for heterochromatin adaptor proteins (HP1) which are involved in the formation of heterochromatin and gene repression. Phosphorylation of histones may also produce similar repressive effects depending on the cellular context. Often, it is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.
References
Product Information
Format
Affinity Purified
Control
HeLa cell extract and recombinant Histone H3 protein
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size, is a rabbit polyclonal antibody for detection of Histone H3 trimethylated at lysine 36 (H3K36me3). This purified Ab is specificity verified by dot blot (DB) and validated in WB, ICC. Small pack size is ideal for screening applications.
Key Applications
Western Blotting
Immunocytochemistry
Dot Blot
Chromatin Immunoprecipitation (ChIP)
ChIP-seq
Application Notes
Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-trimethyl-Histone H3 (Lys36) (1:2,000 dilution).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Histone H3 in A431 cells.
ChIP-Sequencing: Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat#17-10460, 2 µg of anti-trimethyl-Histone H3 (Lys36) antibody(ABE435-S), 20 µL Protein A/G beads , and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq™ instrument. An excess of eleven million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human Histone H3 trimethylated at Lys36.
Epitope
Trimethylated Lys36
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes Histone H3 trimethylated at Lys36.
Species Reactivity
Human
Mouse
Species Reactivity Note
Demonstrated to react with Human and Mouse. Broad species reactivity expected due to similar sequence homology.
~17 kDa observed. An uncharacterized band appears at ~52 kDa in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell extract and recombinant Histone H3 protein.
Western Blot Analysis: A 1:500 dilution of this antibody detected Histone H3 on 10 µg of HeLa cell extract and recombinant Histone H3 protein.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
