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OP09 Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801)

This Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IP, Paraffin Sections for the detection of p53 (Ab-2) (Pantropic).

MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
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OP09
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Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Prezzi e disponibilità

Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
OP09-100UG
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      OP09-200UG
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      Fuori produzione
      Disponibili quantità limitate
      La disponibilità deve essere confermata
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          Prodotti rimanenti: riceverà un nostro avviso
          Will advise
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          Contact Customer Service

          		 Fiala di plastica 200 μg
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          Description
          OverviewRecognizes the ~53 kDa wild-type and mutant p53 protein in A-431 cells and breast carcinoma tissue.
          Catalogue NumberOP09
          Brand Family Calbiochem®
          References
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Negative controlSK-OV-3 cells or normal skin
          Positive controlA431 cells or breast carcinoma tissue
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Application ReferencesOriginal Clone Legros, Y., et al. 1994. Oncogene 9, 2071. Epitope Identification Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.
          Key Applications Frozen Sections
          Gel Shift
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Paraffin Sections
          Application NotesFrozen Sections (5 µg/ml)
          Gel Shift (see comments)
          Immunoblotting (2.5 µg/ml)
          Immunoprecipitation (1 µg/sample)
          Paraffin Sections (5 µg/ml, pepsin, trypsin, or heat pre-treatment required)
          Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53; short incubation times with 35S-Met (≤ 1 h) will help reduce background. For immunoblots of wild-type p53, maximize sensitivity by preconcentrating samples by immunoprecipitation with Cat. No. OP09, then immunoblot using Cat. No. PC35 and chemiluminescent detection. For a gel shift assay, use Cat. No. OP09L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena human p53 fusion protein
          ImmunogenHuman
          Epitopewithin amino acids 46-55 of human p53
          ClonePAb1801
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Numero di catalogo GTIN
          OP09-100UG 04055977225013
          OP09-200UG 04055977225020

          Documentation

          Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) MSDS

          Titolo

          Scheda di sicurezza (MSDS) 

          Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) Certificati d'Analisi

          TitoloNumero di lotto
          OP09

          Riferimenti bibliografici

          Panoramica delle referenze
          El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.

          Brochure

          Titolo
          Caspases and other Apoptosis Related Tools Brochure
          Scheda tecnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-October-2007 RFH
          ApplicationFrozen Sections (5 µg/ml)
          Gel Shift (see comments)
          Immunoblotting (2.5 µg/ml)
          Immunoprecipitation (1 µg/sample)
          Paraffin Sections (5 µg/ml, pepsin, trypsin, or heat pre-treatment required)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.
          BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
          HostMouse
          Immunogen speciesHuman
          Immunogena human p53 fusion protein
          Epitopewithin amino acids 46-55 of human p53
          ClonePAb1801
          IsotypeIgG₁
          Specieshuman, not mouse, not rat
          Positive controlA431 cells or breast carcinoma tissue
          Negative controlSK-OV-3 cells or normal skin
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsWild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53; short incubation times with 35S-Met (≤ 1 h) will help reduce background. For immunoblots of wild-type p53, maximize sensitivity by preconcentrating samples by immunoprecipitation with Cat. No. OP09, then immunoblot using Cat. No. PC35 and chemiluminescent detection. For a gel shift assay, use Cat. No. OP09L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
          Application referencesOriginal Clone Legros, Y., et al. 1994. Oncogene 9, 2071. Epitope Identification Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.

          Prodotti e applicazioni correlate

          Alternative Products

          Numero di catalogo Descrizione  
          OP03 Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Prezzi e disponibilità
          OP33 Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) Prezzi e disponibilità

          Categorie

          Life Science Research > Antibodies and Assays > Primary Antibodies