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OP03L Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)

This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).

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OP03L
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Panoramica

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, Mk, M, Rb, RMMonoclonal Antibody

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OP03L-100UG
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      Description
      OverviewRecognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated p53.
      Catalogue NumberOP03L
      Brand Family Calbiochem®
      References
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Legros, Y., et al. 1994. Oncogene 9, 2071.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
      Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
      Harlow, E., et al. 1981. J. Virol. 39 861.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controlNormal skin tissue or SK-OV-3 cells
      Positive controlBreast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Wang, H., et al. 1991. Cell Growth and Diff. 2, 645. Immunofluorescence, Frozen Sections Davidoff, A.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5006. Immunoprecipitation Lehman, T.A., et al. 1991. Cancer Res. 51, 4090. Harlow, E., et al. 1981. J. Virol. 39, 861. Original Clone Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709. Harlow, E., et al. 1981. J. Virol. 39 861. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071. Waterman, M.J.F., et al. 1998. Nature Genetics 19, 175. (within amino acids 376-378) Cross Reactivity/Phosphophorylated p53/Acetylated p53 Li, M., et al. 2002. J. Biol. Chem. 227, 50607. Delphin, C. and Baudier, J. 1994. J. Biol. Chem. 269, 29579.
      Key Applications Flow Cytometry
      Frozen Sections
      Gel Shift
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesFrozen Sections (10 µg/ml, see application references)
      Gel Shift (see comments)
      Immunoblotting (10 µg/ml, see application references)
      Immunofluorescence (1-20 µg/ml, see application references)
      Flow Cytometry (1-20 µg/ml)
      Immunoprecipitation (1 µg per sample, see application references)
      Application CommentsOriginally called clone L21. Does not recognize phosphorylated or acetylated p53. For a gel shift assay, resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then perform immunoblotting using Cat. No. PC35 and chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified mouse p53
      ImmunogenMouse
      Epitopewithin amino acids 376-378 of human p53
      ClonePAb421
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Monkey
      • Mouse
      • Rabbit
      • Rat
      Antibody TypeMonoclonal Antibody
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide; freezing of aliquots is best for long-term storage of reconstituted product, but repetitive freezing and thawing should be avoided.
      Global Trade Item Number
      Numero di catalogo GTIN
      OP03L-100UG 04055977209594