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MABS2195-100UG Anti-gp91phox (NOX2) Antibody, clone 54.1

Anti-gp91phox (NOX2) clone 54.1, Cat. No. MABS2195, is a mouse monoclonal antibody that detects Cytochrome b-245 heavy chain ( gp91phox) and is tested for use in Flow Cytometry and Western Blotting.

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MABS2195-100UG
100 μg  
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      Replacement Information
      Description
      Catalogue NumberMABS2195-100UG
      DescriptionAnti-gp91phox (NOX2) Antibody, clone 54.1
      Alternate Names
      • Cytochrome b-245 heavy chain
      • CGD91-phox
      • Cytochrome b(558) subunit beta
      • Cytochrome b558 subunit beta
      • Heme-binding membrane glycoprotein gp91phox
      • NADPH oxidase 2
      • Neutrophil cytochrome b 91 kDa polypeptide
      • Superoxide-generating NADPH oxidase heavy chain subunit
      • gp91-1
      • p22 phagocyte B-cytochrome
      Background InformationCytochrome b-245 heavy chain (UniProt: P04839; also known as CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome) is encoded by the CYBB (also known as NOX2) gene (Gene ID: 1536) in Human. Gp91phox is a multi-pass membrane glycoprotein of the NADPH oxidase family that contains three extracellular domains, six transmembrane domains, and four cytoplasmic domains. It contains four iron binding sites and has a ferric oxidoreductase domain that is localized in the amino acids 54-286. It is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide and plays an important role in the host defense function of phagocytic cells. It also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It can undergo polyubiquitination at lysine 48 by RNF145 that triggers its endoplasmic reticulum-associated degradation. Clone 54.1 recognizes the conserved carboxy-terminal domain of NOX family proteins and shows higher specificity for NOX2 and reduced reactivity with NOX3, NOX4, and NOX1 in the descending order. Mutations in CYBB gene are known to cause granulomatous disease that is characterized by the inability of neutrophils and phagocytes to kill ingested life-threatening bacteria and fungus. (Ref.: (Ref.: Burritt, JB., et al. (2003). J. Immunol. 170 (12); 6082-6089; Burritt, JB., et al. (1995). J. Biol. Chem. 270(28):16974-16980).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-gp91phox (NOX2) clone 54.1, Cat. No. MABS2195, is a mouse monoclonal antibody that detects Cytochrome b-245 heavy chain ( gp91phox) and is tested for use in Flow Cytometry and Western Blotting.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesWestern Blotting Analysis: A representative lot detected gp91phox (NOX2) in Western Blotting applications (Baniulis, D., et. al. (2005). Biochim Biophys Acta. 1752(2):186-96; Burritt, J.B., et. al. (2003). J Immunol. 170(12):6082-9).

      Flow Cytometry Analysis: A representative lot detected gp91phox (NOX2) in Flow Cytometry applications (Burritt, J.B., et. al. (2003). J Immunol. 170(12):6082-9).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPartially purified human neutrophil flavocytochrome b588 ( gp91-phox).
      EpitopeC-terminal half
      Clone54.1
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 54.1 is a mouse monoclonal antibody that detects human gp91phox/NOX2. It targets an epitope within 8 amino acids from the C-terminal, fourth cytoplasmic domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CYBB
      • NOX2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~91 kDa observed; 65.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human PMN cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected gp91phox (NOX2) in Human PMN cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABS2195-100UG 04065265872652