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07-030-AF647 Anti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor® 647 Conjugate

This Anti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of dimethyl Histone H3.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FB, HIST1H3C, H3FC, HIST1H3D, H3FD, HIST1H3E, HIST1H3F, H3FF, HIST1H3G, HIST1H3H, H3FH, HIST1H3I, H3FI, HIST1H3J, H3FJ, H3FK, HIST3H3, H3FT, H3T UniProt Number: Q16695
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07-030-AF647
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, TICCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-030-AF647
      DescriptionAnti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor® 647 Conjugate
      Alternate Names
      • H3K4me2
      • H3.3B, Lys4 dimethylated
      • H3 histone, family 3A, Lys4 dimethylated
      • H3 histone, family 3B, Lys4 dimethylated
      • Histone H3, Lys4 dimethylated
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of dimethyl Histone H3.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antiserum (Cat. No. 07-030) is shown to be suitable also for dot blot, chromatin immunoprecipitation (ChIP), ChIP-seq, immunocytochemistry, immunofluorescence, and Western blotting applications.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide (ART[Kme2]QTAR-GC) corresponding to amino acids 1-8 of human histone H3.
      EpitopeH3K4me2
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificitySpecificity of this polyclonal antibody toward histone H3 Lys4 dimethylation was demonstrated by dot blot analysis using the unconjugated antiserum (Cat. No. 07-030).
      Species Reactivity
      • Human
      • Tetrahymena
      Species Reactivity NoteHuman, Tetrahymena. Broad species cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FB
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FD
      • HIST1H3E
      • HIST1H3F
      • H3FF
      • HIST1H3G
      • HIST1H3H
      • H3FH
      • HIST1H3I
      • H3FI
      • HIST1H3J
      • H3FJ
      • H3FK
      • HIST3H3
      • H3FT
      • H3T
      Modifications
      • Methylation
      Purification MethodProtein A purified.
      UniProt Number
      Molecular Weight~17 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys4 dimethylation in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      07-030-AF647 04055977355581