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OP24 Anti-c-K-Ras (Ab-1) Mouse mAb (234-4.2)

OP24
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Panoramica

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

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OP24-100UG
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      Description
      OverviewRecognizes c-K-Ras and v-K-Ras in SW480 and Y1 cells and normal skin tissue. Cross-reacts with c-H-Ras and c-N-Ras.
      Catalogue NumberOP24
      Brand Family Calbiochem®
      References
      ReferencesMizuuchi, H. et al. 1992. Cancer Res. 2, 2777.
      Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
      Sirdransky, D., et al. 1992. Science 256, 102.
      Shaw, P., et al. 1991. Oncogene 6, 2121.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M.E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlSW480 or Y1 cells or normal skin tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Palejwala, S. and Goldsmith, L.T. 1991. BioTechniques 11, 606.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (10 µg/ml)
      Immunoblotting (5 µg/ml with 1% gelatin, see comments and application references)
      Immunoprecipitation (see comments)
      Paraffin Sections (10 µg/ml, saponin or pepsin pre-treatment required)
      Application CommentsThis antibody reacts with c-K-Ras and v-K-Ras, and cross-reacts with c-H-Ras or c-N- Ras by immunoblotting. The level of expression of p21 Ras is variable in different tissues. For this reason we recommend a sample concentration step prior to immunoblotting (see application references). For immunoprecipitation, use 5 µg Cat. No. OP24 per sample with 45 µl protein A agarose. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, rat K-Ras
      ImmunogenRat
      Epitopewithin amino acids 54-189
      Clone234-4.2
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Numero di catalogo GTIN
      OP24-100UG 04055977208368