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MABN1188 Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2

This Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tyrosine Hydroxylase.

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Research Category: Neuroscience Research Sub-Category: Neuronal & Glial Markers Gene Symbol: Th UniProt Number: P04177
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MABN1188
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1188
      ReplacesMAB318
      DescriptionAnti-Tyrosine Hydroxylase Antibody, clone 10F8.2
      Alternate Names
      • TH
      • Tyrosine 3-monooxygenase
      • Tyrosine 3-hydroxylase
      • Tyrosine Hydroxylase
      Background InformationTyrosine 3-monooxygenase (EC 1.14.16.2; UniProt P04177; also known as TH, Tyrosine 3-hydroxylase) is encoded by the Th gene (Gene ID 25085) in rat species. Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines, dopamine and norepinephrine, from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. TH enzymatic activity requires ferrous ions as cofactor and is believed to be regulated by phosphorylation. Six human TH isoforms exist as a result of alternative splicing (UniProt P07101-1 through P07101-6).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bλ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tyrosine Hydroxylase.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected tyrosine hydroxylase in rat brain tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal region sequence of rat tyrosine hydroxylase.
      EpitopeC-terminal region.
      Clone10F8.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 10F8.2 targets a C-terminal epitope present in rat and mouse TH sequence (UniProt P04177 & P24529), as well as in all six human TH spliced isoforms (UniProt P07101).
      IsotypeIgG2bλ
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteHuman, Rat. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Th
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~50-60 kDa observed. 55.83 kDa (rat), 55.86 kDa (mouse), 58.03/55.48/58.47/55.92/45.21/44.77 kDa (human isoform 1/2/3/4/5/6) calculated (Met1 removed). The multiplet banding pattern observed in rat PC12 lysate most likely represent differentially phosphorylated TH species (Gozal, E., et al. (2005). J. Appl. Physiol. 99(2):642-649).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

      Western Blotting Analysis: 0.25 µg/mL of this antibody detected tyrosine hydroxylase in 10 µg of PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABN1188 04055977355437

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      Categorie

      Life Science Research > Antibodies and Assays > Primary Antibodies