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05-511-AF488 Anti-TCF-4 Antibody, clone 6H5-3 Antibody, AlexaFluor® 488 Conjugate

This Anti-TCF-4 Antibody, clone 6H5-3 Antibody, AlexaFluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of TCF-4.

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Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: TCF7L2, TCF4 UniProt Number: Q9NQB0
05-511-AF488
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue Number05-511-AF488
      DescriptionAnti-TCF-4 Antibody, clone 6H5-3 Antibody, AlexaFluor® 488 Conjugate
      Alternate Names
      • Transcription factor 7-like 2
      • HMG box transcription factor 4
      • T-cell-specific transcription factor 4
      • T-cell factor 4
      • TCF-4
      • hTCF-4
      • TCF-4
      Background InformationTranscription factor 7-like 2 (UniProt Q9NQB0; also known as HMG box transcription factor 4, hTCF-4, T-cell factor 4, T-cell-specific transcription factor 4, TCF-4) is encoded by the TCF7L2 (also known as TCF4) gene (Gene ID 6934) in human.
      TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified mouse antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-TCF-4 Antibody, clone 6H5-3 Antibody, AlexaFluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of TCF-4.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TCF-4 in HeLa, HUVEC, and NIH/3T3 cells.
      The unconjugated antibody (Cat. No. 05-511) is shown to be suitable also for Western blotting, Electrophoretic mobility shift assay (EMSA), immunocytochemistry, immunohistochemistry, and immunoprecipitation applications.
      Biological Information
      ImmunogenAmino acids 31-331 of human TCF-4.
      Epitopea.a. 31-331
      Clone6H5-3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TCF7L2
      • TCF4
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~66 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected TCF-4 in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      05-511-AF488 04055977298000