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MABN1792 Anti-Sortilin Antibody, clone F11

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MABN1792
100 μg  
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      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIH(P), ICC, WB, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1792
      DescriptionAnti-Sortilin Antibody, clone F11
      Alternate Names
      • Sortilin
      • 100 kDa NT receptor
      • Glycoprotein 95
      • Gp95
      • Neurotensin receptor 3
      • NT3
      • NTR3
      Background InformationSortilin (UniProt Q99523; also known as 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3) is encoded by the SORT1 (also known as LDLCQ6, NT3) gene (Gene ID 6272) in human. Sortlin belongs to the family of sorting receptors that are characterized by the Vps10p domain, which creates a tunnel cavity with its ten-bladed β-propeller for the binding of soluble ligands. These family members also contain a short cytoplasmic tail that harbors recognition motifs for subcellular sorting adaptor proteins. Sortilin is reported to bind PCSK9 in the trans-Golgi network with high-affinity in HepG2 cells. Sort1 knockout mice exhibit low levels of PCSK9 in plasma and its subcellular localization in hepatocytes is also altered. Sortilin is also found to co-localize with amyloid precursor protein (APP) in neurites and it facilitates APP processing toward production of sAPPα, possibly by optimizing APP as substrate for α-secretase cleavage. Sortilin is initially produced with a signal (a.a. 1-33) and a propeptide (a.a. 34-77) sequence, the removal of which yields the mature receptor (a.a. 78-831) with a large luminal/extracellular (a.a. 78-555) portion, a transmembrane region(a.a. 756-778), and a short cytoplasmic tail (a.a.779-831). Like the other family members (MPRs and SorLA), sortilin resides both on the membrane of intracellular vesicles and on the plasma membrane. Altered sortilin expressions due to SORT1 genetic variations affect serum levels of low density lipoprotein cholesterol (LDL-C) and contribute to the LDL-C level quantitative trait locus 6 (LDLCQ6) and susceptibility to myocardial infarction.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Sortilin Antibody, clone F11 is an antibody against Sortilin Antibody for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blotting and ELISA.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Western Blotting
      • ELISA
      Application NotesImmunocytochemistry Analysis: A representative lot detected cellular localization of endogenous sortilin in isolated hippocampal neurons from new born (P0) wild-type, but not Sort1−/− C57BL/6J mice (Gustafsen, C., et al. (2013). J Neurosci. 33(1):64-71).
      Immunocytochemistry Analysis: Representative lots detected cellular localization of exogenously expressed sortilin in transfected HEK293 cells by fluorescent immunocytochemistry (Larsen, J.V., et al. (2014). Biochem J. 457(2):277-288; Gustafsen, C., et al. (2013). J Neurosci. 33(1):64-71; Larsen, J.V., et al. (2010). Mol. Cell. Biol. 30(17):4175-4187; Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851).
      ELISA Analysis: A representative lot was employed as the detection antibody in sandwich ELISA. A positive correlation was found between PCSK9 and soluble sortilin in human serum samples from healthy individuals (Gustafsen, C., et al. (2014). Cell Metab. 19(2):310-318).
      Western Blotting Analysis: A representative lot detected different distribution of Sortilin than SorLA among subcellular fractions obtained by gradient centrifugation (Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851).
      Biological Information
      ImmunogenRecombinant protein corresponding to the extracellular domain of human Sortilin.
      EpitopeExtracellular domain
      CloneF11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SORT1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~91 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human cerebral cortex tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sortilin in human cerebral cortex tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABN1792 04055977294842

      Documentation

      Anti-Sortilin Antibody, clone F11 MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Anti-Sortilin Antibody, clone F11 Certificati d'Analisi

      TitoloNumero di lotto
      Anti-Sortilin, clone F11 - 3192047 3192047
      Anti-Sortilin, clone F11 - 3280265 3280265
      Anti-Sortilin, clone F11 - 3593691 3593691
      Anti-Sortilin, clone F11 - 3703582 3703582
      Anti-Sortilin, clone F11 - 3868418 3868418
      Anti-Sortilin, clone F11 - 3870080 3870080
      Anti-Sortilin, clone F11 - 3898673 3898673
      Anti-Sortilin, clone F11 - 3934988 3934988
      Anti-Sortilin, clone F11 - 4056325 4056325
      Anti-Sortilin, clone F11 -Q2611290 Q2611290

      Riferimenti bibliografici

      Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
      Human sorCS1 binds sortilin and hampers its cellular functions.
      Larsen, JV; Hermey, G; Sørensen, ES; Prabakaran, T; Christensen, EI; Gliemann, J; Madsen, P; Petersen, CM
      The Biochemical journal  457  277-88  2014

      Mostra il sommario
      24128306 24128306
      The hypercholesterolemia-risk gene SORT1 facilitates PCSK9 secretion.
      Gustafsen, C; Kjolby, M; Nyegaard, M; Mattheisen, M; Lundhede, J; Buttenschøn, H; Mors, O; Bentzon, JF; Madsen, P; Nykjaer, A; Glerup, S
      Cell metabolism  19  310-8  2014

      Mostra il sommario
      24506872 24506872
      Sortilin and SorLA display distinct roles in processing and trafficking of amyloid precursor protein.
      Gustafsen, C; Glerup, S; Pallesen, LT; Olsen, D; Andersen, OM; Nykjær, A; Madsen, P; Petersen, CM
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  64-71  2013

      Mostra il sommario
      23283322 23283322
      Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer.
      Larsen, JV; Hansen, M; Møller, B; Madsen, P; Scheller, J; Nielsen, M; Petersen, CM
      Molecular and cellular biology  30  4175-87  2009

      Mostra il sommario
      20584990 20584990
      Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA.
      Nielsen, MS; Gustafsen, C; Madsen, P; Nyengaard, JR; Hermey, G; Bakke, O; Mari, M; Schu, P; Pohlmann, R; Dennes, A; Petersen, CM
      Molecular and cellular biology  27  6842-51  2007

      Mostra il sommario
      17646382 17646382

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      Categorie

      Life Science Research > Antibodies and Assays > Primary Antibodies