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04-1016 Anti-STAT5α (CT) Antibody, clone E289, rabbit monoclonal

Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: MGF, STAT5 UniProt Number: P42229
04-1016
100 µL  
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      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, ICC, IH(P), IP, FCRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1016
      DescriptionAnti-STAT5α (CT) Antibody, clone E289, rabbit monoclonal
      Alternate Names
      • signal transducer and activator of transcription 5A
      Background InformationSTAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases. In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT. Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand. After dimerization, the STATs accumulate in the nucleus by way of nuclear transport. STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production. The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.
      Product Information
      FormatUnpurified
      Control
      • A431 cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Stat5α in human squamous lung carcinoma tissue.
      Immunocytochemistry Analysis: A 1:100 dilution from a previous lot was used in IC.
      Flow Cytometry: A 1:10 dilution of a previous lot was used in flow cytometry.
      Immunoprecipitation Analysis: A 1:80 dilution from a previous lot was used in IP.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues in C-terminus of human STAT5α.
      EpitopeC-terminus
      CloneE289
      HostRabbit
      SpecificityThe antibody does not cross-react with other STAT family members.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MGF
      • STAT5
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
      SUBUNIT STRUCTURE: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 By similarity. Interacts with NCOA1 and SOCS7.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation.
      PTM: Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity By similarity.
      SEQUENCE SIMILARITIES: Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
      Molecular Weight~92 kDa
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on A431 cell lysates.
      Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect STAT5α in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Numero di catalogo GTIN
      04-1016 04053252472756