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05-417 Anti-Paxillin Antibody, clone 5H11

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05-417
250 µg  
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      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, B, AvICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-417
      Replaces04-581
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Paxillin Antibody, clone 5H11
      Alternate Names
      • FLJ16691
      • paxillin
      Background InformationPaxillin, a focal adhesion scaffolding protein, is phosphorylated on multiple tyrosine residues in response various stimulations including integrin stimulation, growth factor stimulation, and oncogenic transformation (v-Src, v-Crk and BCR/ABL). Paxillin binds many other focal adhesion proteins including FAK, Vinculin, Talin, and Tensin. As such, Paxillin and it tyrosine phosphorylation, help regulate the dynamics of the focal adhesions and the actin cytoskeleton which results in cell morphology and cell migration characteristics of the cell. Paxillin is most notable phosphorylated on tyrosines 31 and 118 (Tyr31 and Tyr118). Interestingly, Vinculin and FAK bind to overlapping sites on Paxillin. Additionally, there is an increase in Paxillin phosphorylation at Tyr118 in vinculin (-/-) and Vinculin Y822F cells. Paxillin Y31F Y118F expression restores apoptosis and inhibits Erk activity.
      Product Information
      FormatPurified
      Control
      • Included Positive Antigen Control:
        Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Store at -20°C.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Paxillin Antibody, clone 5H11 is an antibody against Paxillin for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 μg of a previous lot of antibody immunoprecipitated paxillin from 500 μg of a 3T3/A31 RIPA lysate.

      Immunocytochemistry:
      5-10 μg/mL of a previous lot gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7% paraformaldehyde for 5 minutes.
      Biological Information
      ImmunogenGST fusion protein containing fulllength human paxillin. Clone 5H11.
      Clone5H11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes paxillin.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      • Avian
      Species Reactivity NoteHuman, mouse, rat, chicken and bovine. Other species cross-reactivity not tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PXN
      • Paxillin
      • paxillin
      • FLJ16691
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P49023 # Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
      SIZE: 591 amino acids; 64533 Da
      SUBUNIT: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.
      PTM: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
      SIMILARITY: SwissProt: P49023 ## Belongs to the paxillin family. & Contains 4 LIM zinc-binding domains.
      Molecular Weight68 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on RIPA lysates from 3T3/A31 fibroblasts.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected paxillin in RIPA lysates from 3T3/A31 fibroblasts.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size250 µg
      Global Trade Item Number
      Numero di catalogo GTIN
      05-417 04053252729126