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MABS831-25UG Anti-PI3 Kinase Antibody, p85 Antibody, N-SH3 Antibody, clone 1D5.1

Anti-PI3 Kinase, p85, N-SH3, clone 1D5.1, Cat. No. MABS831, is a mouse monoclonal antibody that detects human and murine p85 subunit of PI3 kinase. It has been tested for use in Western Blotting.

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Research Category: Signaling Gene Symbol: PIK3R1, GRB1 UniProt Number: P27986
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MABS831-25UG
25 μg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS831-25UG
      DescriptionAnti-PI3 Kinase Antibody, p85 Antibody, N-SH3 Antibody, clone 1D5.1
      Alternate Names
      • Phosphatidylinositol 3-kinase regulatory subunit alpha
      • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
      • PI3-kinase regulatory subunit alpha
      • PtdIns-3-kinase regulatory subunit p85-alpha
      • PI3-kinase subunit p85-alpha
      • PI3K regulatory subunit alpha
      • PtdIns-3-kinase regulatory subunit alpha
      Background InformationPhosphatidylinositol 3-kinase regulatory subunit alpha (UniProt: P27986; also known as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha) is encoded by the PIK3R1 (also known as GRB1) gene (Gene ID: 5295) in human. PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Following ligand binding, tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. PI3 kinase alpha plays a role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Mutations in PIK3R1 gene are known to be associated with insulin resistance.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-PI3 Kinase, p85, N-SH3, clone 1D5.1, Cat. No. MABS831, is a mouse monoclonal antibody that detects human and murine p85 subunit of PI3 kinase. It has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected PI3 Kinase, p85, N-SH3 in 10 µg of human cerebral cortex tissue lysate.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 77 amino acids from the N-terminal region of the p85 regulatory subunit of human PI 3-kinase alpha.
      Clone1D5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1D5.1 detects p85 subunit of human and murine PI-3 kinase alpha. It targets an epitope within 77 amino acids from the N-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PIK3R1
      • GRB1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~85 kDa observed; 83.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 L1 cell lysate.
      Western Blotting Analysis: 0.5 µg/mL of this antibody detected PI3 Kinase, p85, N-SH3 in 10 µg of NIH/3T3 L1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABS831-25UG 04054839553684