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ABN2288-25UL Anti-Nesprin-2/SYNE2

Anti-Nesprin-2/SYNE2, Cat. No. ABN2288, is a rabbit polyclonal antibody that detects Nesprin-2 and is tested for use in Immunocytochemistry and Western Blotting.

ABN2288-25UL
25 µL  
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      Replacement Information
      Description
      Catalogue NumberABN2288-25UL
      DescriptionAnti-Nesprin-2/SYNE2
      Alternate Names
      • KASH domain-containing protein 2
      • KASH2
      • Nuclear envelope spectrin repeat protein 2
      • Nucleus and actin connecting element protein
      • Protein NUANCE
      • Synaptic nuclear envelope protein 2
      • Syne-2
      Background InformationNesprin-2 (UniProt: Q6ZWQ0; also known as KASH domain-containing protein 2, KASH2, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, Protein NUANCE, Synaptic nuclear envelope protein 2, Syne-2) is encoded by the Syne2 gene (Gene ID: 319565) in murine species. Nesprin-2 is a single-pass type IV membrane protein with a large cytoplasmic domain (aa 1-6823), a transmembrane domain (aa 6824-2844), and a perinuclear space domain (aa 6845-6874). It contains two calponin-homology domains (aa 31-136 and 183-288) and fifty-six spectrin repeats. Its actin-binding region is localized in amino acids 1-286. Nesprin-2 is a multi-isomeric modular protein that forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. It is a core component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex that is involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Three different isoforms of Nesprin-2 have been described that are produced by alternative splicing. Different isoform patterns are found in the different compartments of the cell. Nesprin-2 levels are shown to be down-regulated in cancer cells. Nesprin-2 knockdown cells exhibit a defective nuclear translocation of c-Fos and Smad 2/3. Mutations in Syne2 gene have been linked to Emery-Dreifuss muscular dystrophy 5 (EDMD5), an autosomal dominant form of degenerative myopathy. (Ref.: Zhang, Q., et a; (2019). Curr. Biol. 29(17); 2826-2839; Kelkar, P., et al. (2015). Nucleus. 6(6); 479-489; Luxton, GWG., et al. (2010). Science. 329(5994); 956-959).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Nesprin-2/SYNE2, Cat. No. ABN2288, is a rabbit polyclonal antibody that detects Nesprin-2 and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesTested applications

      Western Blotting Analysis: A representative lot detected Nesprin-2/SYNE2 in Western Blotting application (Luxton, G.W., et. al. (2010). Science. 329(5994); 956-9).

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Nesprin-2/SYNE2 in NIH-3T3 overexpressing Nesprin-2.

      Immunocytochemistry Analysis: A representative lot detected Nesprin-2/SYNE2 in immunocytochemistry application (Zhang, Q., et. al. (2019). Curr Biol. 29(17); 2826-2839).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 284 amino acids from the N-terminal, actin-binding region of mouse Nesprin-2.
      EpitopeN-terminal
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Nesprin-2. It targets an epitope within the N-terminal, actin-binding region.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Canine.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Syne2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~135 kDa observed for Nesprin-2 fragment; 782.75 kDa calculated for whole protein. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in NIH3T3 cells.

      Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Nesprin-2/SYNE2 in NIH3T3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      ABN2288-25UL 04065269802419