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MABE340 Anti-MDM2 Antibody, clone IF2

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MABE340
100 µg  
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      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE340
      DescriptionAnti-MDM2 Antibody, clone IF2
      Alternate Names
      • E3 ubiquitin-protein ligase Mdm2
      • Double minute 2 protein
      • Hdm2
      • Oncoprotein Mdm2
      • p53-binding protein Mdm2
      Background InformationMDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes the transcriptional activity of p53 in two main ways. The N-terminal of MDM2 interacts and disables the transactivation domain of p53. In addition, the C-terminal E3 ligase region of MDM2 can attach monoubiquitin to p53 resulting in the transport of p53 from the nucleus to the cytoplasm, thereby preventing the interaction of p53 with target DNA sequences. In addition, MDM2 may also attach polyubiquitin chains to p53, resulting in the degradation of p53 by the proteosome. MDM2 may produce these effects in cooperation with the Mdmx protein. Overall, MDM2 inhibits the ability of p53 to induce cell cycle arrest or apoptosis, and may contribute to oncogenesis.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • A431 cell lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MDM2 Antibody, clone IF2 is a Mouse Monoclonal Antibody for detection of MDM2 also known as E3 ubiquitin-protein ligase Mdm2 or Hdm2 & has been validated in WB & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: A representative lot detected MDM2 in Raji, HepG2, and Molt-4 cell lysate.

      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MDM2 in human prostate cancer tissue.
      Biological Information
      ImmunogenRecombinant protein corresponding to human MDM2.
      CloneIF2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MDM2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~85 kDa and ~60 kDa observed. Uniprot describes many isoforms between 12-56 kDa, which can be highly phosphorylated and ubiquitinated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected MDM2 in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABE340 04053252522253

      Documentation

      Anti-MDM2 Antibody, clone IF2 MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Anti-MDM2 Antibody, clone IF2 Certificati d'Analisi

      TitoloNumero di lotto
      Anti-MDM2, clone IF2 -2786917 2786917
      Anti-MDM2, clone IF2 - 2366584 2366584
      Anti-MDM2, clone IF2 - 3166084 3166084
      Anti-MDM2, clone IF2 - 3205918 3205918
      Anti-MDM2, clone IF2 - 3235641 3235641
      Anti-MDM2, clone IF2 - 3243414 3243414
      Anti-MDM2, clone IF2 - 3273631 3273631
      Anti-MDM2, clone IF2 - 3321075 3321075
      Anti-MDM2, clone IF2 - 3438605 3438605
      Anti-MDM2, clone IF2 - 3490106 3490106

      Riferimenti bibliografici

      Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
      A phosphatidylinositol 3-kinase/Akt pathway promotes translocation of Mdm2 from the cytoplasm to the nucleus.
      Mayo, L D and Donner, D B
      Proc. Natl. Acad. Sci. U.S.A., 98: 11598-603 (2001)  2001

      Mostra il sommario
      11504915 11504915

      Brochure

      Titolo
      New Products: Volume 3, 2012

      Newsletters / Publications

      Title
      Research Focus - Volume 5 2012

      Prodotti e applicazioni correlate

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      Categorie

      Life Science Research > Antibodies and Assays > Primary Antibodies