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MABN88 Anti-K+/Cl- Cotransporter (KCC2) Antibody, clone N1/12

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MABN88
100 µg  
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      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, RIHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN88
      DescriptionAnti-K+/Cl- Cotransporter (KCC2) Antibody, clone N1/12
      Alternate Names
      • Electroneutral potassium-chloride cotransporter 2
      • Erythroid K-Cl cotransporter 2
      • Neuronal K-Cl cotransporter
      • electroneural potassium-chloride cotransporter 2
      • solute carrier family 12
      • potassium-chloride transporter), member 5
      Background InformationKCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
      References
      Product Information
      FormatPurified
      Control
      • Rat cortex area A-3 tissue
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect K+/Cl-Cotransporter (KCC2) using this Anti-K+/Cl-Cotransporter (KCC2) Antibody, clone N1/12 validated for use in IH.
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: 1:300 dilution from a previous lot detected KCC2 in rat hypothalamus tissue.
      Biological Information
      ImmunogenRecombinant protein corresponding to rat KCC2.
      EpitopeUnknown
      CloneN1/12
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes KCC2
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with rat. Shown by an independent laboratory to react in human and mouse tissue. (UC Davis/NIH NeuroMab Facility Department of Neurobiology, Physiology and Behavior)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNeuronal specific potassium-chloride cotransporter isoform; may play a role in response to neuronal injury [RGD].
      Gene Symbol
      • SLC12A5
      • KIAA1176
      • hKCC2
      • KCC2
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl- homeostasis in neurons. Necessary for the ontogenic change in response to gamma-aminobutyric acid (GABA) from depolarization to hyperpolarization during neuronal development.

      SUBUNIT STRUCTURE: Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1 (By similarity).

      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

      TISSUE SPECIFICTY: Highly expressed in brain. Not detected in other tissues. Highly expressed in pyramidal neurons and in neurons throughout the cortex, hippocampus, the granular layer of the cerebellum and in groups of neurons throughout the brainstem. Barely detectable in dorsal-root ganglions.

      DEVELOPMENTAL STAGE: Detected in thalamus, but not in hippocampus and neocortex at E20. At birth barely detectable in hippocampus. Expression increases steeply from day 5 to day 9 and then stabilizes at adult levels.

      SEQUENCE SIMILARITIES: Belongs to the SLC12A transporter family.
      Molecular Weight 126 kDa Calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in rat cortex area A-3 tissue.

      Immunohistochemistry Analysis: 1:300 dilution of this antibody detected KCC2 in rat cortex area A-3 tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABN88 04053252412875

      Documentation

      Anti-K+/Cl- Cotransporter (KCC2) Antibody, clone N1/12 MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Anti-K+/Cl- Cotransporter (KCC2) Antibody, clone N1/12 Certificati d'Analisi

      TitoloNumero di lotto
      Anti-K+/Cl- Cotransporter (KCC2), -2802594 2802594
      Anti-K+/Cl- Cotransporter (KCC2), clone N1/12 - 2332591 2332591
      Anti-K+/Cl- Cotransporter (KCC2), clone N1/12 - 2445061 2445061
      Anti-K+/Cl- Cotransporter (KCC2), clone N1/12 - 1966920 1966920
      Anti-K+/Cl- Cotransporter (KCC2), clone N1/12 - 2192288 2192288
      Anti-K+/Cl- Cotransporter (KCC2), clone N1/12 - 3174819 3174819
      Anti-K+/Cl- Cotransporter (KCC2), clone N1/12 - 3314349 3314349
      Anti-K+/Cl- Cotransporter (KCC2), clone N1/12 - 3589760 3589760
      Anti-K+/Cl- Cotransporter (KCC2), clone N1/12 - 3911117 3911117
      Anti-K+/Cl- Cotransporter (KCC2), clone N1/12 - 4048714 4048714

      Riferimenti bibliografici

      Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
      Alterations in Brain Inflammation, Synaptic Proteins, and Adult Hippocampal Neurogenesis during Epileptogenesis in Mice Lacking Synapsin2.
      Chugh, D; Ali, I; Bakochi, A; Bahonjic, E; Etholm, L; Ekdahl, CT
      PloS one  10  e0132366  2015

      Mostra il sommario
      26177381 26177381

      Brochure

      Titolo
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.